肺癌RASSF1A基因甲基化与cyclin D1的表达

目的探讨肺癌中RASSFIA基因甲基化与cyclinD1表达对肺癌诊断的意义及RASSF1A基因甲基化与cyclinD1表达两者之间有无相关性。方法甲基化特异性PCR检测33例肺癌及10例癌旁组织中RASSFIA基因启动子区甲基化情况；用免疫组织化学S-P法检测cyclinD1在上述组织中的表达情况。分析两者分别与患者年龄、性别、组织分型的关系及与肿瘤分化间的相关性，同时对RASSFIA基因甲基化与cyclinD1表达间的关系进行分析。结果33例肺癌中有18例（54．5％）显示RASSFIA基因已被甲基化，16例（48．5％）肺癌组织显示为cyclinD1表达阳性；而10例癌旁组织RASSFIA基因未甲基化且cyclinD1免疫组化染色显示为阴性。RASSFIA基因甲基化与年龄、性别、组织分型、分化及cyclinD1表达均无关；而cyclinDl表达与肿瘤的分化具有相关性（r=0．418，P〈0．05），但与年龄、性别、组织分型无关（P〉0．05）。结论RASSFlA基因甲基化与cyclinD1表达在肺癌的发生中具有重要作用，且cyclinD1表达与肿瘤分化有关，检测cyclinD1表达有助于对肺癌恶性程度作出判断。

RASSF1A methylation and the expression of cyclin D1 in lung cancer

Objective To investigate the value of RASSF1A promoter methylation and cyclin D1 expression in lung cancer diagnosis,and analyse the relativity between RASSF1A promoter methylation and the expression of cyclin D1.Methods Methylation-specific PCR(MSP)was performed for detecting the methylation status of RASSF1A promoters in 33 cases of lung cancer and 10 paracancerous normal lung tissue samples.Immunohistochemiscal S-P method was used to detect the expression of cyclin D1 in all the samples above.Results 18 lung cancer cases(54.5%)showed that their RASSF1A gene were methylated,and cyclin D1 expressed in 16 out of 33(48.5%) lung cancer samples.but no RASSF1A gene methylation were detected in the 10 paracancerous normal lung tissue samples,and immunohistochemical staining of these samples for cyclin D1 was negative.Either RASSF1A methylation or cyclin D1 expression had no relationship with gender,age,and histology type.The expression of cyclin D1 had correlation with differentiation of lung cancer(r=0.418,P<0.05),but no relationship was found between RASSF1A gene methylation and cyclin D1 expression in lung cancer.Conclusion RASSF1A methylation and overexpression of cyclin D1 play important role in carcinogenesis of lung.The expression of cyclin D1 has correlation with the differentiation of lung cancer,and can be used to judge the degree of malignancy of lung cancer.