The effect of 3-methylindole on the uptake and incorporation of14C-choline into phospholipids in lung tissue slices |
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Authors: | James B Kirkland Tammy M Bray |
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Affiliation: | (1) Department of Nutrition, University of Guelph, N1G 2W1 Guelph, Ontario, Canada |
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Abstract: | 3-Methylindole (3MI) is the causative agent in the development of acute bovine pulmonary edema. Microscopic studies revealed
a structural disruption in the lamellar bodies of type II cells, indicating an abnormal metabolism of phospholipid in the
lung of 3MI treated animals. In the present study, lung slices from 4 goats were used to investigate the changes in phosphatidylcholine
metabolism induced by 3MI. Eighteen slices were cut from each healthy lung and divided into control and 3MI groups. After
a 4-hr pretreatment with 3MI (.19 or .57 mM) or carrier, the level of incorporation of14C-choline into phosphatidylcholine, sphingomyelin and their water soluble intermediates was studied. The uptake of14C-choline and its incorporation into phosphatidylcholine and sphingomyelin was depressed by 3MI treatment. In the water soluble
fractions, the radioactivity increased in free choline and CDP-choline while it decreased in P-choline. This suggests that
choline kinase and the P-choline transferases have become relatively more rate limiting and may play a role in the depressed
de novo synthesis of phosphatidylcholine induced by 3MI. |
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