老年白血病患者Survivin基因表达及临床意义

目的探讨Survivin基因在老年白血病患者白血病细胞中的表达,并分析与预后的关系。方法应用RT-PCR对32例不同时期老年急性白血病（AL）患者及5例慢性淋巴细胞白血病患者的白血病细胞Survivin基因mRNA表达情况进行检测,以10例健康人作为对照。结果 32例老年AL患者白血病细胞Survivin mRNA阳性表达率为71.87%,急性淋巴细胞白血病（ALL）细胞Survivin mRNA表达率为71.42%与急性非淋巴细胞白血病（ANLL）细胞76%的表达率比较差异无统计学意义,二者均高于对照组（χ2=5.90、6.81,P〈0.05）。初治和复发AL患者白血病细胞Survivin mRNA阳性表达率分别为78.01%、84.15%,明显高于对照组（χ2=12.61、9.23,P〈0.05）,老年慢性淋巴细胞白血病患者的白血病细胞中无Survivin表达。结论老年急性白血病的发生、发展可能与Survivin基因的过度表达密切相关。

The Survivin Gene Expression of Elderly Patients with Leukemia and Clinical Significance

Objective To investigate the survivin gene expression in leukemia cells of elderly patients with leukemia,and analysis the factors of prognostic.Methods 32 patients with different stages of acute leukemia（AL）and 5 patients with chronic lymphocytic leukemia were divided into observation group,10 healthy people were divided into control group.The expression of survivin mRNA in leukemia cells was detected by RT-PCR.Results The expression of Survivin mRNA was 71.87% in acute leukemia（AL）cells,the expression of Survivin mRNA was 71.42% in acute lymphoblastic leukemia（ALL）cells,and the expression of Survivin mRNA was 76% in acute non-lymphocytic leukemia（ANLL）cells,the differences had no statistical significance,but the expression of Survivin mRNA were higher than the control group（χ^2 = 5.90,6.81,P〈0.05）.The expression of Survivin mRNA was 78.01% and 84.15% in leukemia cells of newly diagnosed and relapsed acute leukemia patients,significantly higher than the control group（χ^2=12.61,9.23,P〈0.05）.Leukemia cells without Survivin expression in Elderly patients with chronic lymphocytic leukemia.Conclusion The incidence and development of acute leukemia may be closely related to overexpression of Survivin gene.