siRNA抑制nm23-H1基因表达对鼻咽癌6-10B细胞生物学行为的影响

目的：采用RNA干扰技术下调nm23-H1基因在鼻咽癌6-10B细胞中的表达，探讨nm23-H1表达下调对6-10B细胞生物学行为的影响。方法：采用脂质体法将nm23-H1基因siRNA(nm23-H1 siRNA)瞬间转染6-10B细胞，Western 印迹检测转染细胞中nm23-H1蛋白的表达水平，然后利用MTT法、流式细胞术和Transwell小室实验分别检测转染6-10B细胞的增殖、细胞周期和体外迁移侵袭等生物学行为的变化；测序检测6-10B细胞nm23-H1基因有无S120G 点突变。结果： nm23-H1 siRNA有效地下调nm23-H1基因的表达， nm23-H1 siRNA转染6-10B细胞的增殖能力增强，S期细胞增多，体外迁移和侵袭能力增强（P<0.05）。6-10B细胞nm23-H1 基因无S120G 点突变。结论：nm23-H1基因具有抑制人鼻咽癌细胞6-10B增殖和体外迁移侵袭的作用。

Effect of nm23-H1 siRNA on biological behavior of human nasopharyngeal carcinoma cell line 6-10B

Objective To silence nm23-H1 gene expression in nasopharyngeal carcinoma (NPC) cell line 6-10B cells by siRNA, and to explore the effect of nm23-H1 downregulation on the biological behavior of 6-10B cells. MethodsThe nm23-H1 siRNAs and control siRNA were transiently transfected into 6-10B cells by liposome transfection method, respectively. Western blot was used to detect nm23-H1 expression in the transfected cells. MTT assay and flow cytometry were performed to determine the cell growth and cell cycle distribution in the transfected cells, respectively. Transwell system was performed to detect the migration and invasion of the transfected cells. DNA sequencing was used to detect the S120G mutation of nm23-H1 gene in the 6-10B cells. ResultsNm23-H1 siRNA could effectively downregulate the expression of nm23-H1 in 6-10B cells. nm23-H1 downregulation could enhance the cell growth, and increase the number of S phase cells and the ability of the in vitro cell migration and invasion in the 6-10B cell line (P< 0．05). S120G mutation of nm23-H1 gene was not found. Conclusion Nm23-H1 gene behaves as a metastasis suppressor gene.