软骨链蛋白在小鼠体内分布和代谢的示踪研究

目的 研究软骨链蛋白125I的标记和在小鼠体内的分布与代谢，为该蛋白质在肿瘤、软骨、结缔组织疾病以及地方病等领域的基础研究提供方法学指导和代谢参数.方法 氯胺-T法标记软骨链蛋白，亲和凝胶层析法分离标记物进行体内示踪研究.结果 软骨链蛋白标记率为89.90%，放射化学纯度为92.77%,样品比活度为8.13×109Dpm/μg，该蛋白与透明质酸的最大结合率为89.23%.平均血浆半减期为1.84小时,血浆清除率为1.75ml/h,排除速率为10.03ml/h,稳态双库代谢模型为：Ct=2.4986e-0.5635t+3.24e-0.2833t.结论 为临床相关疾病的研究提供实验指导和代谢参数.

The Labeling of Cartilage Link Protein and Its Distribution and Metabolism Study

Objective The cartilage link protein (CLP) was labeled by125Iodine.Then the labeled CLP was traced in mice.In order to create a simple and easy experimental method,and to get metabolism paramters for study of tumors,cartilage,connective tissue disease and endemic et al.Methods CLP was labeled with125NaI by the chloramine T and was separated with gel and affinity chromatography.The125I-CLP was examined by in vivo biodistribution and metabolism study.Results Radioiodine labelling rate was 89.90%,radiochemical purity was 92.77%,specific activity was 8.13×109 Dpm/μg and maximal binding rate of125I-CLP to hyaluronic acid was 89.23%.Average plasma half life (T1/2) is 1.84 hour. Clearance rate 1.75 ml/hour.Exclusion rate is 10.03 ml/hour. Steady double metabolic pool model is Ct = 2.4986e-0.5635t+3.24e-0.2833t.Conclusions An effective method of 125I-labeling cartilage link protein is esablished.The cartilage link protein may distribute on the body exist in extracellular ligquid.Liver metabolizes it mainly.Experimental method and metabolism paramters were provided for correlative clinical diseases.