Copper–4,4′-dipyridyl coordination compound as solid reagent for spectrophotometric determination of reducing sugar employing a multicommutation approach |
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| Affiliation: | 1. Departamento de Química Fundamental, Universidade Federal de Pernambuco, 50670-901 Recife, Pernambuco, Brazil;2. Centro de Tecnologias Estratégicas do Nordeste, 50670-901 Recife, Pernambuco, Brazil;3. Instituto Federal de Alagoas, Campus Coruripe, 57035 Maceio, Al, Brazil;4. Departamento de Química, Universidade Federal Rural de Pernambuco, 52171-900 Recife, PE, Brazil;1. Departamento de Química Inorgánica, Universidade de Vigo, 36310 Vigo, Spain;2. CFisUC, Department of Physics, University of Coimbra, Rua Larga, 3004-516 Coimbra, Portugal;3. Department of Chemistry, University of Coimbra, Rua Larga, 3004-535 Coimbra, Portugal;4. Unit of Biophysics, CIMAGO, IBILI, Faculty of Medicine, University of Coimbra, Azinhaga de Santa Comba, Celas, 3030-548 Coimbra, Portugal;1. Heritage High School, Rogers, AR 72756, USA;2. Lyman Briggs College and Department of Chemistry, Michigan State University, East Lansing, MI 48825 USA;1. Department of Inorganic and Analytical Chemistry, University of Debrecen, H-4010 Debrecen, Hungary;2. Istituto di Chimica Biomolecolare, CNR, Traversa La Crucca 3, Regione Baldinca, 07040 Li Punti (SS), Italy;3. MTA-DE Homogeneous Catalysis and Reaction Mechanisms Research Group, University of Debrecen, H-4010 Debrecen, Hungary;1. The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, Jiangsu, China;2. State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, Jiangsu, China |
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| Abstract: | In this work a multicommuted flow system employing copper–4,4′- dipyridyl coordination compound as the solid-phase reagent for the spectrophotometric determination of reducing sugar was developed. The coordination compound was synthesized through a reaction of the 4,4′-dipyridyl and copper (II) nitrate, under hydrothermal conditions. The complex was characterized by infrared spectroscopy (FTIR), power X-ray diffraction (XRD), scanning electron microscopy (SEM), energy dispersive spectrometer (EDS) and thermogravimetric analysis (TGA). Based on the characterization, a multicommuted spectrophotometric procedure for the determination of reducing sugar using copper (II) complex as solid reagent is proposed. The proposed method was based on the redox reaction between a monosaccharide, such as fructose and glucose (reducing sugar) and Cu(II). This reaction, mediated in an alkaline medium, produces a yellow compound that can be determined by absorption electronic spectroscopy (λABS = 420 nm). Under optimum experimental conditions, a linear response ranging from 1.0 to 20.0 g L?1 (R = 0.9978 and n = 5), a detection (3σ criterion) and quantification (10σ criterion) limit estimated at 0.23 and 0.75 g L?1, respectively, a standard deviation relative of 4.7% (n = 7), for a reference solution of 10.0 g L?1 reducing sugar, and a sampling rate of 75 determinations per hour were achieved. The proposed system was applied to the determination of reducing sugars in coconut water and juices. The analysis of ten samples and the application of the t-test to the results found, and those obtained using reference procedures (AOAC), provided no significant differences at a 95% confidence level. This system enabled the analysis of reducing sugar with ease and simplicity, providing a significant economy of the solid reagent (600 μg per determination) and reducing effluent generation. |
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| Keywords: | Multicommutation Solid reagent Reducing sugars Coordination compound Solid phase reactor |
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