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| 利用深黄被孢霉发酵生产油脂的初步研究 | |
| 引用本文： | 耿青伟,吴开云,吴小芹,李纪元,刘明靖,平秀敏.利用深黄被孢霉发酵生产油脂的初步研究[J].林业科学研究,2010,23(3):336-341. |
| 作者姓名： | 耿青伟 吴开云 吴小芹 李纪元 刘明靖 平秀敏 |
| 作者单位： | 1. 中国林业科学研究院亚热带林业研究所,浙江,富阳,311400;南京林业大学森林资源与环境学院,江苏,南京,210037 2. 中国林业科学研究院亚热带林业研究所,浙江,富阳,311400 3. 南京林业大学森林资源与环境学院,江苏,南京,210037 4. 云南农业大学农业与生物技术学院,云南,昆明,650201 |
| 基金项目： | 浙江省科技计划重大项目(2006C12030) |
| 摘 要： | 为提高微生物油脂产量,以产油微生物深黄被孢霉为试验菌株,采用单因素试验设计,通过摇瓶培养,研究了产脂培养基中碳源、氮源种类及其浓度、接种量、初始pH值、无机盐离子对菌体生长和油脂积累的影响,确定了深黄被孢霉摇瓶发酵产油脂的优化培养条件为:葡萄糖100 g.L-1,酵母粉3.0 g.L-1,接种量为20%,pH值为5.0~6.0,硫酸镁(MgSO4.7H2O)0.5 g.L-1,磷酸二氢钾(KH2PO4)2.0 g.L-1。在优化培养条件下菌体生物量为20.62 g.L-1,油脂含量为43.02%,油脂产量为8.87 g.L-1。 |
| 关 键 词： | 深黄被孢霉 发酵 油脂 |
Preliminary Study on the Mortierella isabellina Fermentating to Produce Lipid |
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| GENG Qing-wei,WU Kai-yun,WU Xiao-qin,LI Ji-yuan,LIU Ming-jing and PING Xiu-min.Preliminary Study on the Mortierella isabellina Fermentating to Produce Lipid[J].Forest Research,2010,23(3):336-341. | |
| Authors: | GENG Qing-wei WU Kai-yun WU Xiao-qin LI Ji-yuan LIU Ming-jing and PING Xiu-min |
| Affiliation: | Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Fuyang 311400,Zhejiang,China;;College of Forest Resources and Environment, Nanjing Forestry University, Nanjing 210037, Jiangsu, China;;Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Fuyang 311400,Zhejiang,China;;College of Forest Resources and Environment, Nanjing Forestry University, Nanjing 210037, Jiangsu, China;;Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Fuyang 311400,Zhejiang,China;;Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Fuyang 311400,Zhejiang,China;;Faculty of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming 650201, Yunnan, China) |
| Abstract: | To improve the yield of microbial lipids, the lipids production was studied by using the fungus Mortierella isabellina. On the basis of the single factor experiment, the effect of various carbon and nitrogen sources and their concentrations, inoculum size, initial pH and inorganic ions on mycelium growth and accumulation of lipid yield in shaker culture was studied. The results showed that the optimal culture condition were as follows: glucose 100 g·L-1, yeast extract 3.0 g·L-1, inoculum size 20%, initial pH 5.0 - 6.0, MgSO4·7H2O 0.5 g·L-1, KH2PO4 2.0 g·L-1.Under the optimum culture condition, the biomass yield was 20.62 g·L-1, lipid content was 43.02%, the lipid yield was 8.87 g·L-1. |
| Keywords: | Mortierella isabellina ferment lipid |
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