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发酵-渗透汽化连续耦合合成ABE的动力学
引用本文:张俊青,陈春燕,崔海娣,李伟佳,应超,石尔,肖泽仪.发酵-渗透汽化连续耦合合成ABE的动力学[J].精细化工,2012,29(9):866-869.
作者姓名:张俊青  陈春燕  崔海娣  李伟佳  应超  石尔  肖泽仪
作者单位:1. 四川大学化学工程学院,四川成都,610065
2. 长沙理工大学能源与动力工程学院,湖南长沙,410076
基金项目:国家自然科学基金项目(20776088);湖南省教育厅资助项目(08C114);湖南省科技厅资助项目(2008SK4025)~~
摘    要:采用PDMS膜生物反应器和丙酮丁醇梭菌进行了生产ABE的封闭循环连续发酵实验,研究了发酵和渗透汽化分离连续耦合条件下的发酵动力学行为。发酵-分离连续耦合实验运行持续时间长达192 h。运行过程中,细胞质量浓度维持在0.84~4.00 g/L,发酵液中ABE的总质量浓度为5.14~17.54 g/L,葡萄糖质量浓度大约为16.08~35.15 g/L,总体积产率为0.36 g/(L.h)。结果表明,膜生物反应器系统运行稳定,发酵-渗透汽化分离连续耦合生产ABE的操作模式具有可行性和优越性。

关 键 词:ABE发酵  渗透汽化  动力学  丙酮丁醇梭菌  生物工程

Kinetics of ABE Synthesis by Continuous Coupling of Fermentation with Pervaporation
ZHANG Jun-qing , CHEN Chun-yan , CUI Hai-di , LI Wei-jia , YING Chao , SHI Er , XIAO Ze-yi.Kinetics of ABE Synthesis by Continuous Coupling of Fermentation with Pervaporation[J].Fine Chemicals,2012,29(9):866-869.
Authors:ZHANG Jun-qing  CHEN Chun-yan  CUI Hai-di  LI Wei-jia  YING Chao  SHI Er  XIAO Ze-yi
Affiliation:1 (1.School of Chemical Engineering,Sichuan University,Chengdu 610065,Sichuan,China;2.School of Energy and Power Engineering,Changsha University of Science and Technology,Changsha 410076,Hunan,China)
Abstract:Continuous coupling of acetone-butanol-ethanol(ABE) fermentation with pervaporation was conducted in a continuous and closed-circulating fermentation system by combining Clostridium acetobutylicum and PDMS membrane bioreactor.The continuous fermentation successfully lasted for 192 h,and the corresponding kinetic behaviors were studied.During the continuous fermentation,the cell mass concentrations were kept within the range of 0.84~4.00 g/L,the glucose mass concentrations remained within the range of 16.08~35.15 g/L,and the total solvents mass concentrations in the fermentation broth were within 5.14~17.54 g/L.Under such conditions,ABE productivity was 0.36 g/(L·h).The results show that ABE fermentation continuously coupling with pervaporation in a PDMS membrane bioreactor system is feasible and promising.
Keywords:ABE fermentation  pervaporation  kinetics  Clostridium acetobutylicum  biological engineering
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