| 中华鳖不同组织Sox基因表达的RTPCR分析 |
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| 引用本文： | 聂刘旺,单祥年,汪鸣.中华鳖不同组织Sox基因表达的RTPCR分析[J].激光生物学报,2000,9(3):181-184. |
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| 作者姓名： | 聂刘旺 单祥年 汪鸣 |
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| 作者单位： | [1]安徽师范大学生物系,中国安徽芜湖 [2]南京师范大学生物系,中国江苏南京 |
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| 基金项目： | 安徽省和江苏省自然科学基金资助项目!( 974 1 2 0 0 2 and 9750 61 1 2 ) |
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| 摘 要： | 本文采用ＲＴ－ＰＣＲ技术，研究了中华鳖不同组织Ｓｏｘ基因的表达，并通过ＰＣＲ直接克隆法，分析了来自睾丸、脑和脾组织中的Ｓｏｘ基因序列。结果表明：在中华鳖的成本组织中，Ｓｏｘ基因在脑、心肌、肾、脾和雄性的睾丸组织中均有不同程度的表达，而在肌肉、肝脏和雌性的卵巢中则无表达，显示该基因具有组织表达特异性。克隆分析显示，在睾丸组织中表达的是ＴＳＳｏｘ１和ＴＳＳｏｘ４基因，而在脑组织中表达的是ＴＳＳｏｘ２和
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| 关 键 词： | 中华鳖 RT－PCR 序列分析 基因表达 |
| 修稿时间： | 2000-05-09 |
The
RTPCR Expression Analysis on Sox Gene of
Different Tissues from the Trionyx Sinensis |
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| NIE Liuwang ,SHAN Xiangnian ,WANG Ming ,GUO Chaowen ,LU Xiaoxuan.The
RTPCR Expression Analysis on Sox Gene of
Different Tissues from the Trionyx Sinensis[J].ACTA Laser Biology Sinica,2000,9(3):181-184. |
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| Authors: | NIE Liuwang SHAN Xiangnian WANG Ming GUO Chaowen LU Xiaoxuan |
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| Affiliation: | NIE Liuwang 1,SHAN Xiangnian 2,WANG Ming 1,GUO Chaowen 1,LU Xiaoxuan 2 |
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| Abstract: | In the present paper, the Sox gene expression analysis of different tissues from the Trionyx sinensis was studied by using RTPCR, and Sox gene fragments of expression from the testicle, brain and spleen were cloned using RTPCR products. The results show that Sox gene has specific expression in the testicle, brain, spleen, cardiac muscle and kindney and hasn't expression in muscle, liver and ovary. The results of clone reveal that the Sox genes of expression in the testicle are TSSox1 and TSSox 4, and those are TSSox2 and TSSox4 in the brain, and that is TSSox4 in the spleen. This suggests that the Sox gene act important role not only on the sex determination, but also on the development of neural system, immunocyte system and the differentiattion of male germ cell. |
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| Keywords: | Trionyx sinensis RTPCR Sequence analysis Gene expression |
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