同种异体移植瘤在不同组织微环境中生物学行为的差异及机制

 目的 分析不同的组织微环境对同种异体移植瘤生物学行为的影响因素,并探讨基质金属蛋白酶和转化生长因子β₁与移植瘤生物学行为的关系. 方法 应用常规HE染色观察不同移植瘤的生物学行为表现,并应用免疫组化的方法分别检测两种瘤组织中MMP-2,MMP-9及TGF-β₁的表达情况. 结果 ①不同的组织微环境同种异体移植瘤生物学行为有明显差别,皮下转移瘤表现为明显的膨胀性生长;腹腔转移瘤表现为显著的浸润性生长;②MMP-2及MMP-9的表达:皮下转移瘤两者均为阴性;腹腔转移瘤可见MMP-2及MMP-9阳性表达;③TGF-β₁的表达:皮下转移瘤可见TGF-β₁的间质型表达;腹腔转移瘤可见TGF-β₁的胞浆型表达. 结论 不同组织环境对同种移植瘤的浸润及转移有影响,MMP-2、MMP-9及TGF-β₁表达可能是其中的因素之一.

The difference and mechanism of identical transplant-tumors' biological deed in different organic micro-environment

Objective 　To study the effect elements about different organic micro-environment to and investigate the relationship between MMPs , TGF-β1 and transplant-tumor’s biological deed. Methods 　The staining with HE method is conducted to investigate the presentation of different transplant-tumor’s biological deed. Immunohistochemistry is used to detect the expression of MMP-2 , MMP-9 , TGF-β1in two tumor organ respectively. Results 　①The biological deeds of identical transplant-tumor′s from different body have significant difference in different organic micor-envionment . subcutaneous metastaic carcinoma presents expensive grouth ; Belly metastaic carcinoma presents infilt rative grouth. ②The expression of MMP-2 and MMP-9 : Subcutaneous metastaic carcinoma is negative ; belly metastaic carcinoma is positive. ③The expression of TGF-β₁: In subcutaneons metastaic carcinoma , TGF-β₁ is expressed in interstitial tissue. In belly metastaic carcinoma , TGF-β₁ is expressed in cytoplasm. Conclusion 　Different organicmicro-environment will affect the metastasis and infilt ration of identical transplant-tumor. The expression of MMP-2 , MMP-9 and TGF-β₁ is one of it s elements.