慈菇蛋白酶抑制剂对亚洲玉米螟消化酶的影响

亚洲玉米螟幼虫中肠消化酶主要为胰蛋白酶：利用慈菇蛋白酶抑制剂研究它对玉米螟消化酶的影响，发现这种抑制剂体外强烈抑制玉米螟幼虫中肠消化酶：同时我们又比较了长时间取食（三龄至五龄第二天）和短时间取食(四龄未取食48小时)抑制剂的幼虫体内胰蛋白酶活性，当它们短时间取食抑制剂后，体内消化酶水平迅速下降，取食更长时间时，胰蛋白酶活力下降更明显，仅为对照的27．2%。由此可见，慈菇蛋白酶抑制剂严重影响了玉米螟的正常消化，阻断了玉米螟营养与食物间的联系．使玉米螟生长发育不良。

THE EFFECT OF INGESIED ARROWHEAD PROTEINASE INHIBITOR ON DIGESTIVE ENZYME OF ASIAN CORN BORER,OSTRINIA FURNACALIS

Evlbence was presented that Asian corn borer,O strinia furnacalis,depended ontrypsin for protein digestion. We studied the effect of AHI on the larval digestive physiology us- ing a artificial diet with AHI at 0.4%(d.wt )level. The in vitro inhibition of AHI on the activi- ty of trypsin isolated from larval borers was g reat.We also examined both acute and chronic ef- fects of ingestion of AHI on the level of digestive enzyme activity in the larval borer. We foundthat acute ingestion of AHI by the larvae resulted in a siginmcant decrease in tryptic activitywhen compared with insects feeding on control diets. When the larvae chronically were ingestedwith AHI,their tryptic activity was extremely reduced to 27.2%of control.These results sug-gest that AHI seriously affects the normal digestion of Asian corn borer and hinders relationshipbetween the larval nutrition and food resulting in harmful effects on the larval growth and devel- opment.