不同品种菊花和贵州产野菊花中木犀草素的含量比较

目的测定并比较不同品种菊花和贵州产野菊花中木犀草素的含量。方法用高效液相色谱法测定木犀草素的含量。色谱柱为ODS柱（4．6mm×250mm，5μm），流动相为甲醇-水-冰醋酸（体积比45：55：0．4），检测波长为254nm，流速1．0mL／min。结果7个菊花样品的木犀草素含量，以贵州野菊花的木犀草素含量最高（3．876mg／g），杭菊的木犀草素含量最低（0．302mg／g）。结论贵州产野菊花中木犀草素的含量均高于其他品种菊花，具有较高的药用价值。

Content Comparison of Luteolin from Chrysanthemum morifolium of Different Varieties and Chrysanthemum indicum from Guizhou

Objective To determine and compare luteolin content from Chrysanthemum morifolium of different varieties and Chrysanthemum indicum from Guizhou. Methods The HPLC method was established to determine the content of luteolin. The separation was performed on a ODS column（4.6 mm × 250 mm,5 μm） and the mobile phase consisted of methanol, water and glacial acetic acid in the ratio of 45：55：0.4 （v/v）. The UV detector wavelength was set at 254 nm with a flow rate of 1.0 mL/min. Re- suits In the seven Chrysanthemum samples the highest luteolin content was found in Chrysanthemum indicum from Guizhou with 3. 876 mg/g. The lowest one was found in Chrysanthemum morifolium from Hangzhou with 0.302 mg/g. Conclusion The luteolin content and the medicinal value of Chrysanthemum indicum from Guizhou are higher than that of other Chrysanthemum samples.