不同rag基因型牙龈卟啉单胞菌在慢性牙周炎患者中的分布

目的分析不同rag基因型牙龈卟啉单胞菌在慢性牙周炎患者中的分布状况。方法收集50例慢性牙周炎患者的150个龈下菌斑样本，采用16S rDNA 聚合酶链反应（PCR）法检测牙龈卟啉单胞菌在牙周炎病变位点的检出率，并根据各rag基因型的特异性引物检测牙龈卟啉单胞菌不同rag基因型在慢性牙周炎病变位点的分布。结果经16S rDNA PCR法检测，病变位点牙龈卟啉单胞菌阳性检出率为70.7%。各rag基因型在牙龈卟啉单胞菌阳性位点的总检出率：rag-1为60.4%，rag-2为23.6%，rag-3为44.3%，rag-4为15.1%；经统计学检验，rag-1和rag-3型检出率较高，高于rag-2和rag-4型（P<0.05）。结论慢性牙周炎患者龈下菌斑中的牙龈卟啉单胞菌存在rag基因多态性，rag-1和rag-3基因型牙龈卟啉单胞菌与中国辽宁地区人群慢性牙周炎的发生发展关系密切。

Distribution of rag genotypes of Porphyromonas gingivalis in patients with chronic periodontitis

Objective To investigate the distribution of rag genotypes of Porphyromonas gingivalis（P.gingivalis） in chronic periodontitis patients. Methods Subgingival plaque samples were collected from 50 chronic periodontitis patients. The occurrence of P.gingivalis was determined by polymerase chain reaction（PCR） using 16S rDNA-specific primers. Distribution of rag genotypes was assessed in P.gingivalis positive samples by PCR. Results The occurrence of P.gingivalis was 70.7%, and the distribution of four rag genotypes among P.gingivalis positive samples was as follows: rag-1 60.4%; rag-2 23.6%; rag-3 44.3%; rag-4 15.1%, respectively. Conclusion P.gingivalis with various rag genotypes was present in subgingival plaque samples from chronic periodontitis patients, and P.gingivalis with rag-1 and rag-3 were more predominant in chronic periodontitis patients, which may be associated with the development of periodontitis.