| 双纸片协同试验在检测产超广谱β-内酰胺酶细菌中的临床应用 |
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| 引用本文： | 熊薇.双纸片协同试验在检测产超广谱β-内酰胺酶细菌中的临床应用[J].华中科技大学学报(医学版),2001,30(3):285-288. |
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| 作者姓名： | 熊薇 |
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| 作者单位： | 华中科技大学同济医学院附属同济医院检验科, |
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| 摘 要： | 用双纸片协同试验检测我院近6个月来临床标本中产ESBLS的细菌，并探讨双纸片协同试验的最佳方法。结果显示，在244株大肠埃希菌、肺炎克雷伯菌和阴沟肠杆菌中产ESBLS菌49株，总检出率为19.67%，另外在嗜麦芽窄食单胞菌检出5例产ESBLS株，铜绿假单胞菌，产气肠杆菌和枸橼酸杆菌各检出1例产ESBLS株，说明我院产ESBLS菌株产生较广泛。由于ESBLS阳性株对临床常用抗生素的耐药率远远高于ESBLS阴性株，使临床治疗面临极大的困难，必须引起临床医生和临床微生物室的重视。提示我院双纸片协同试验的最佳底物是FTX、ATM和FEP，最适距离是25mm，该法与Etest确证试验的阳性符合率为100%，因此该法仍为临床微生物室检测ESBLS的适宜、简便、经济的方法。
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| 关 键 词： | 双纸片协同试验 ESBLS 抗生素耐药性 |
| 修稿时间： | 2000年4月12日 |
The Clinical Use of Double-paper Synergy Test in the Detection of Bacteria Producing Extended-spectrum β-lactamase |
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| Xiong Wei.The Clinical Use of Double-paper Synergy Test in the Detection of Bacteria Producing Extended-spectrum β-lactamase[J].Journal of Huazhong University of Science and Technology(Health Sciences),2001,30(3):285-288. |
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| Authors: | Xiong Wei |
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| Affiliation: | Xiong Wei Department of Laboratory,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030 |
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| Abstract: | The optimal condition of double-paper synergy test in the detection of bacteria producing extended-spectrum β-lactamase (ESBLS) was studied. In 244 isolates of Enteric bacilli，Klebsiella pneumonia，Enteric cloacae，there were 49 ESBLS-producing strains with the positive rate being 19.67 %. Five ESBLS-producing strains were detected in Pseudomonas maltophilla. One ESBLS-producing strains was detected in Pseudomonas aeruginose，Enteric aerogenes and Citrobacter，respectively. The optimal substrates of double-paper synergy test were cefotaxime，aztreonam and cefepime，and the interspace was 25 mm. This method was still a simple，economical and appropriate method in the detection of ESBLS-producing isolates. |
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