A fluorescence scanning electron microscope |
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| Authors: | Takaaki Kanemaru Kazuho Hirata Shin-ichi Takasu Shin-ichiro Isobe Keiji Mizuki Shuntaro Mataka Kei-ichiro Nakamura |
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| Affiliation: | 1. Morphology and Core Unit, Kyushu University Hospital, Kyushu, Japan;2. Department of Anatomy and Cell Biology, Graduate School of Medical Sciences, Kyushu University, Higashi-ku, Maidashi 3-1-1, Fukuoka 812-8582, Japan;3. Advanced Technology Division, JEOL Ltd., Tokyo, Japan;4. Department of Applied Chemistry and Biochemistry, Faculty of Engineering, Kyushu Sangyo University, Fukuoka, Japan;5. Department of Nanoscience, Faculty of Engineering, Sojo University, Kumamoto, Japan;6. International Science Technology Co., Ltd, Kasuga Laboratory, Fukuoka, Japan;g Department of Anatomy, Kurume University School of Medicine, Kurume, Japan |
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| Abstract: | Fluorescence techniques are widely used in biological research to examine molecular localization, while electron microscopy can provide unique ultrastructural information. To date, correlative images from both fluorescence and electron microscopy have been obtained separately using two different instruments, i.e. a fluorescence microscope (FM) and an electron microscope (EM). In the current study, a scanning electron microscope (SEM) (JEOL JXA8600 M) was combined with a fluorescence digital camera microscope unit and this hybrid instrument was named a fluorescence SEM (FL-SEM). In the labeling of FL-SEM samples, both Fluolid, which is an organic EL dye, and Alexa Fluor, were employed. We successfully demonstrated that the FL-SEM is a simple and practical tool for correlative fluorescence and electron microscopy. |
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| Keywords: | Correlative microscopy Scanning electron microscopy Fluorescence microscopy Organic EL fluorophore |
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