| 复方七芍降压片含药血清对大鼠血管平滑肌细胞增殖的影响 |
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| 引用本文： | 王顺民,陆朵梅,黄露,唐路军,陈橙,杨晓丹,姚福胜,张稳.复方七芍降压片含药血清对大鼠血管平滑肌细胞增殖的影响[J].中医药导报,2020,26(1):34-38. |
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| 作者姓名： | 王顺民 陆朵梅 黄露 唐路军 陈橙 杨晓丹 姚福胜 张稳 |
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| 作者单位： | 湖南中医药大学第一附属医院,湖南长沙410007;长沙市第九医院,湖南 长沙410018;湖南航天医院,湖南长沙410006;湖南省中医药研究院,湖南长沙410006;湖南中医药大学,湖南长沙410208 |
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| 基金项目： | 国家自然科学基金;湖南省中医药科研重点项目;湖南省科技厅技术创新引导计划 |
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| 摘 要： | 目的:观察复方七芍降压片含药血清对炎症因子及其诱导的血管平滑肌细胞(VSMC)增殖的影响。方法:采用自发高血压大鼠模型灌喂蒸馏水、复方七芍降压片和厄贝沙坦,分别制备对照血清和含药血清。使用炎症因子TNF-α、嘌呤受体P2Y6抑制剂MRS2578和含药血清对大鼠VSMC进行干预,检测不同处理下大鼠VSMC的细胞活力和细胞周期以及炎症因子IL-6、IL-1β的表达差异。结果:TNF-α可促进细胞增殖,促进IL-6和IL-1β表达;MRS2578可抑制细胞增殖,抑制IL-6和IL-1β表达;复方七芍降压片含药血清和厄贝沙坦含药血清可抑制细胞增殖,抑制IL-6和IL-1β表达。结论:复方七芍降压片含药血清能抑制炎症因子诱导的VSMC增殖,抑制细胞炎症因子的表达。复方七芍降压片降低血压的功效可能与抑制VSMC炎症反应有关。
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| 关 键 词： | 复方七芍降压片 血管平滑肌细胞 细胞增殖 炎症反应 大鼠 |
Effect of Serum with Fufang Qishao Jiangya Tablet(复方七芍降压片)on Proliferation of Vascular Smooth Muscle Cells in Rat |
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| WANG Shun-min,LU Duo-mei,HUANG Lu,TANG Lu-jun,CHEN Cheng,YANG Xiao-dan,YAO Fu-sheng,ZHANG Wen.Effect of Serum with Fufang Qishao Jiangya Tablet(复方七芍降压片)on Proliferation of Vascular Smooth Muscle Cells in Rat[J].GUIDING JOURNAL OF TCM,2020,26(1):34-38. |
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| Authors: | WANG Shun-min LU Duo-mei HUANG Lu TANG Lu-jun CHEN Cheng YANG Xiao-dan YAO Fu-sheng ZHANG Wen |
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| Affiliation: | (l.The First Affiliated Hospital of Hunan University of Chinese Medicine,Changsha Hunan 410007,China;The Ninth Hospital of Changsha,Chdngsha Hunan 410018,China;Hunan Aerospace Hospital,Changsha Hunan 410006.China;Hunan Academy of Traditional Chinese Medicine,Changsha Hunan 410006,China;Hunan University of Chinese Medicine,Changsha Hunan 410208,China) |
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| Abstract: | Objective:To explore the effect of serum with Fufang Qishao Jiangya tablet on the proliferation of vascular smooth muscle cells(VSMC)induced by TNF-αand the expression of inflammatory factors.Methods:Distilled water,Fufang Qishao Jiangya tablet and irbesartan were fed into spontaneously hypertensive rat models to prepare serum control,serum with Fufang Qishao Jiangya tablet and serum with irbesartan.Inflammatory factors TNF-α,Purine receptor P2Y6 inhibitor MRS2578,serum with FHT and serum with irbesartan were used to intervene in rat VSMC.Cell viability,cell cycle and expression of inflammatory factors IL-6 and IL-1βin rats VSMC were detected.Results:TNF-αpromoted cell proliferation and the expression of IL-6 and IL-1β;MRS2578 inhibited cell proliferation and the expression of IL-6 and IL-1β;Serum with FHT and serum with irbesartan inhibited cell proliferation and the expression of IL-6 and IL-1β.Conclusion:Serum with Fufang Qishao Jiangya tablet inhibits the proliferation of VSMC induced by inflammatory factors,and inhibits the expression of inflammatory factors.The effect of Fufang Qishao Jiangya tablet on lowering blood pressure may be related to inhibiting the inflammatory reaction of VSMC. |
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| Keywords: | Fufang Qishao Jiangya tablet vascular smooth muscle cells cell proliferation inflammatory response rat |
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