| 用中国仓鼠V_(79)细胞HGPRT位点正向突变试验检测饮用水的致突变性 |
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| 引用本文： | 吴惠霞,关碧玮,蔡宏道.用中国仓鼠V_(79)细胞HGPRT位点正向突变试验检测饮用水的致突变性[J].华中科技大学学报(医学版),1986(4). |
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| 作者姓名： | 吴惠霞 关碧玮 蔡宏道 |
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| 作者单位： | 同济医科大学卫生系环境微生物学教研室
(吴惠霞，关碧玮)，同济医科大学卫生系环境微生物学教研室(蔡宏道) |
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| 摘 要： | 用国产H-103树脂吸附的武汉D水厂水源水与出厂水的非挥发性、非极性有机浓集物,在不加代谢活化系统条件下,均能诱发中国仓鼠胚胎肺成纤维细胞(V79)次黄嘌呤鸟嘌呤磷酸核糖转移酶(HGPRT)位点的正向突变。出厂水2、4、6升/瓶三种浓度的致突变率分别为空白对照的4.25、14.68、22.75倍;水源水4升/瓶浓度对细胞也有致突变性。提示武汉D水厂的水源水与出厂水中存在着对哺乳动物细胞的直接致突变物,且出厂水中致突变物的活性更强。
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| 关 键 词： | 致突变试验 V_(79)细胞 HGPRT位点 饮用水 |
Detecting Mutagenicity in Drinking Water by Using Chinese Hamster Cells V79/HGPRT Locus Test |
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| Wu Huixia,Guan Biwei,Cai Hongdao.Detecting Mutagenicity in Drinking Water by Using Chinese Hamster Cells V79/HGPRT Locus Test[J].Journal of Huazhong University of Science and Technology(Health Sciences),1986(4). |
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| Authors: | Wu Huixia Guan Biwei Cai Hongdao |
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| Affiliation: | Wu Huixia,Guan Biwei,Cai HongdaoDepartment of Environmental Microbiology,School of PublicHealth,Tongji Medical University,Wuhan |
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| Abstract: | Raw water and its tap water of the Wuhan D Lake were concentrated by non-polar resin H-103 (domestic product).The mutagenicity of such non-volatile water concentrates could be detected by means of Chinese hamster embryo lung fibroblasts V79 cells in HGPRT (Hypoxanthine-guanine phosphoribosyl transferase) locus forward mutagenesis test without activation.Mutagenic activities of the tap water in 2,4,6 liter/bottle concentration were respectively 4.25,14.68 and 22.75 times of the control.Mutagenic activity was also existed in raw water in 4 liter/bottle concentration but less active than the tap water.These results suggested that the raw and its tap water of the Wuhan D Lake possess some direct-acting mutagens for mammalian cells and its mutagenicity is more active in tap water than in raw water. |
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| Keywords: | mutagenesis V_(79) cells HGPRT locus drinking water |
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