红豆杉细胞培养生产紫杉醇产量稳定性的探讨

通过磷酸盐双饥饿和秋水仙碱这两种经典的同步化方法处理悬浮培养的红豆杉细胞 ,以实现培养物的均一性 ,并比较了同步化与非同步化细胞及不同同步化方法处理的细胞紫杉醇产量。结果表明 ,不同同步化方法处理的细胞紫杉醇产量有差异 :秋水仙碱同步处理处于中期的细胞紫杉醇产量高于非同步化细胞 ,而磷酸盐双饥饿同步处理处于间期的细胞紫杉醇产量则相反。这表明紫杉醇产量与培养物的均一性有关 ,且与细胞同步的周期时相有关 ,采用同步化方法来选择合适的细胞周期时相有利于紫杉醇产量的稳定 ,通过比较不同同步化方法处理对细胞生物量和 POD活性的影响进一步探讨紫杉醇产量产生差异的原因

Research on the stability of taxol yield of Taxus chinensis suspension cultures

Two classical synchronization methods, double phosphate starvation and colchicines, were adopted to realize homogeneity of Taxus chinensis suspension cultures, and the taxol yields of cells treated with these different methods were compared. The results showed that different synchronization methods resulted in different taxol yields. After being treated with colchicine, cells at metaphase had higher taxol yield than that of asynchronous cells, however, after being treated with double phosphate starvation, cells at G1 phase had the contrary result. That indicated that taxol yield had relativity with the homogeneity of culture cells, and also had close relativity with cell cycle phase. Therefore, to select suitable cell cycle phase by use of synchronization methods is important for stable taxol production. In addition the effects of different synchronization methods on the biomass and POD activity were investigated to analyze the reasons for difference of taxol yield.