羧甲基壳聚糖包裹椰子油脂质体制备的工艺优化

通过薄膜-超声分散法制备羧甲基壳聚糖包裹的椰子油脂质体,以大豆卵磷脂和氢化大豆卵磷脂为膜材,包封率为主要评价指标,采用单因素和正交实验对薄膜-超声分散法制备羧甲基壳聚糖包裹的椰子油脂质体的工艺和配方进行优化,并对所制备脂质体的微观形态、包封率、丙二醛(malondialdehyde,MDA)含量和体外释放度进行分析。结果表明:最佳制备条件为羧甲基壳聚糖与磷脂比为1:16,磷脂与胆固醇比为8:1,椰子油与磷脂比为3:1,pH为4.5,超声强度750 W,超声时间20 min,此条件下实际包封率为96.94%±0.20％。羧甲基壳聚糖包裹椰子油脂质体在0.9%氯化钠中,24 h的释放度为41.28%±0.30%。椰子油脂质体的包封率高和累积释放度高,稳定性好,可实现延缓释放和靶向给药。

Optimization of preparation process of carboxymethyl chitosan coated coconut oil liposomes

The coconut oil liposomes were prepared by film dispersion method. Soybean lecithin and hydrogenated soybean lecithin were used as membrane material, and the entrapment efficiency was the main evaluation index. Based on one-factor-at-a-time experiments, orthogonal experimental design was applied to optimize process conditions for the liposomes of the coconut oil. The optimal process conditions were determined as follows:carboxymethyl chitosan and lecithin ratio of 1:16, lecithin and cholesterol ratio of 8:1, coconut oil and lecithin ratio of 3:1, pH4.5, 750 W ultrasonic power and 20 min encapsulation time. Under the optimal conditions, the observed value of encapsulation rate was 96.94%±0.20%.The final product carboxymethyl chitosan coated coconut oil liposomes in 0.9% sodium chloride, 24 h release was 41.28%±0.30%. The encapsulation efficiency and cumulative release of coconut oil liposomes are high, stability is good. It can achieve delayed release and targeted administration.