山慈菇多糖的免疫调节作用及对小鼠骨肉瘤细胞S180体内生长抑制作用

目的：探讨山慈菇多糖的免疫调节作用及对小鼠骨肉瘤细胞S180的抑制作用。方法：噻唑蓝法及吞噬中 性红法测定小鼠脾淋巴细胞增殖能力及脾巨噬细胞活性；体内肿瘤抑制实验检测山慈菇多糖对S180荷瘤小鼠肿瘤 生长、脾脏指数和胸腺指数的影响；流式细胞术及酶联免疫吸附测定法检测山慈菇多糖对S180荷瘤小鼠T淋巴细胞 亚群及血清细胞因子水平的影响。结果：山慈菇多糖可以增强淋巴细胞增殖能力和巨噬细胞活性；同时，山慈菇多 糖也可以抑制S180荷瘤小鼠肿瘤生长，增加小鼠脾脏指数和胸腺指数；此外，山慈菇多糖还可以增加荷瘤小鼠CD4 ＋、CD8＋ T细胞浓度，提高CD4＋/CD8＋比值及白细胞介素-2、肿瘤坏死因子-α、干扰素-γ水平。结论：山慈菇 多糖具有明显的肿瘤抑制作用，其对肿瘤细胞生长的抑制作用与提高免疫能力有关。

Effects of Pseudobulbus cremastrae seu pleiones Polysaccharide on the Regulation of Immune Function and the Inhibition of Tumor Growth in Sarcoma S180 Tumor-Bearing Mice

Objective: To explore the effects of Pseudobulbus cremastrae seu pleiones polysaccharide (PCSPP) on the regulation of immune functionand the inhibition of tumor growth in sarcoma S180 tumor-bearing mice. Methods: Lymphocyte proliferation capacity and the phagocytic activity of spleen macrophages were determined by 3-(4,5-dimethyl- 2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide and neutral red methods; the effects of PCSPP on tumor growth, spleen index and thymus index in S180 tumor-bearing mice were evaluated through tumor suppression test in vivo, and the effects on T lymphocyte subgroup and serum cytokine levels were detected by flow cytometry and enzymelinked immunosorbent assay, respectively. Results: PCSPP could enhance lymphocyte proliferation capacity and macrophage phagocytic activity. PCSPP could inhibit tumor growth and increase spleen index and thymus index in S180 tumor-bearing mice; meanwhile, it could also increase the levels of CD4+ and CD8+ T cells in mice and elevate the CD4+/CD8+ ratio, interleukin-2, tumor necrosis factor-α and interferon-γ levels. Conclusion: PCSPP has a potent antitumor effect and its inhibitory effect may be related to ithe improvement of immune function.