| 加工环境中单核细胞增生性李斯特菌耐药特征分析 |
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| 引用本文： | 吴立婷,吴新宇,庞茂达,何涛,包红朵,周艳,孙利厂,王冉,张辉.加工环境中单核细胞增生性李斯特菌耐药特征分析[J].食品安全质量检测技术,2018,9(7):1496-1500. |
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| 作者姓名： | 吴立婷 吴新宇 庞茂达 何涛 包红朵 周艳 孙利厂 王冉 张辉 |
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| 作者单位： | 江苏省食品质量安全重点实验室-省部共建国家重点实验室培育基地江苏省农业科学院;扬州大学生物科学与技术学院 |
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| 基金项目： | 国家自然科学基金项目(31671955)；江苏省农业科技自主创新资金项目(cx(16)1028) |
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| 摘 要： | 目的分析在流通过程中加工环境来源的食源性李斯特菌耐药性及耐药基因携带情况,探讨加工环境对该菌的影响。方法收集2016~2017年分离自加工环境中的71株单核细胞增生性李斯特菌,利用微量肉汤稀释法对8种抗菌药物进行药物敏感性分析,并采用PCR鉴定11种耐药基因(tet A、tet M、tet S、erm A、erm B、erm C、mec A、aac(6’)-Ib、van A、van B、cfr)。结果 71株Lm对庆大霉素(11.27%)、氨苄西林(4.22%)、头孢他啶(98.59%)、环丙沙星(97.18%)、四环素(21.12%)、红霉素(15.49%)、林可霉素(92.96%)均具有不同程度的耐药性,对万古霉素敏感。其中以耐受3~6种抗菌药为主,耐药情况较为严重。耐药基因检测表明四环素类tet A,tet M为主要耐药基因,检出率高达50%以上,其次为红霉素类erm A、erm B、erm C,检出率约11%,氨基糖苷类aac(6’)-Ib的检出率为5.63%。结论加工环境单核细胞增生性李斯特菌的耐药性呈上升趋势,其耐药表型与耐药基因型并不完全一致,从而表明加工环境中存在影响单核细胞增生性李斯特菌耐药性因素,同时为食源性李斯特菌病的防治及预警提供参考依据。
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| 关 键 词： | 单核细胞增生性李斯特菌 耐药性 耐药基因 |
| 收稿时间： | 2018/1/12 0:00:00 |
| 修稿时间： | 2018/3/14 0:00:00 |
Antimicrobial resistance of Listeria monocytogenes isolated from processing environment |
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| WU Li-Ting,WU Xin-Yu,PANG Mao-D,HE Tao,Bao Hong-Duo,ZHOU Yan,SUN Li-Chang,WANG Ran and ZHANG Hui.Antimicrobial resistance of Listeria monocytogenes isolated from processing environment[J].Food Safety and Quality Detection Technology,2018,9(7):1496-1500. |
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| Authors: | WU Li-Ting WU Xin-Yu PANG Mao-D HE Tao Bao Hong-Duo ZHOU Yan SUN Li-Chang WANG Ran and ZHANG Hui |
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| Affiliation: | Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base of Ministry of Science and Technology,Jiangsu Academy of Agricultural Sciences,College of Biological Sciences and Technology,Yangzhou University,Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base of Ministry of Science and Technology,Jiangsu Academy of Agricultural Sciences,Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base of Ministry of Science and Technology,Jiangsu Academy of Agricultural Sciences,Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base of Ministry of Science and Technology,Jiangsu Academy of Agricultural Sciences,Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base of Ministry of Science and Technology,Jiangsu Academy of Agricultural Sciences,Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base of Ministry of Science and Technology,Jiangsu Academy of Agricultural Sciences,Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base of Ministry of Science and Technology,Jiangsu Academy of Agricultural Sciences and Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base of Ministry of Science and Technology,Jiangsu Academy of Agricultural Sciences |
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| Abstract: | |
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| Keywords: | Listeria monocytogenes drug-resistance drug-resistant gene |
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