不同等级道路的运输振动对哈密瓜贮藏过程中抗氧化系统的影响

不同道路对哈密瓜贮藏过程中抗氧化系统的影响均不同，因此本实验模拟半挂车在高速公路、一级公路、 二级公路以及三级公路的运输振动环境，比较经过不同道路振动处理与未经振动处理的哈密瓜在室温（23 ℃）贮 藏期间（28 d）呼吸速率、腐烂指数、总酚含量、VC含量、抗坏血酸过氧化物酶活力、超氧化物歧化酶活力、过 氧化氢酶活力、过氧化物酶活力以及Fe3＋还原能力、1,1-二苯基-2-三硝基苯肼自由基清除能力的变化情况。结果表 明：贮藏28 d时，不同等级道路振动后的哈密瓜7 种指标（总酚含量、VC含量、抗坏血酸过氧化物酶活力、超氧化 物歧化酶活力、过氧化氢酶活力、过氧化物酶活力以及Fe3＋还原能力、1,1-二苯基-2-三硝基苯肼自由基清除能力） 均低于对照组，其中二级公路及三级公路相对于高速公路和一级公路对哈密瓜呼吸作用和腐烂率的促进以及抗氧化 系统的破坏更为显著（P＜0.05）；说明振动会促进哈密瓜贮藏期间的呼吸作用，降低抗氧化保护系统中各种酶 及抗氧化活性，从而加速细胞膜脂氧化，破坏细胞膜完整性，最终加快哈密瓜的衰老与腐败，其中二级公路和 三级公路的运输振动更为显著。

Effect of Transportation Vibration on Different Grades of Road on Antioxidant System of Hami Melons (Cucumis melo var. saccharinus) during Storage

The effects of transportation on different grades of roads on the antioxidant system of Hami melon are different. The transportation vibration environment of a semi-trailer on expressway, arterial road (AR), secondary roads (SR), and tertiary roads (TR) were simulated in this experiment. The respiration rate, decay index, total phenolics content, VC content, ascorbate peroxidase (APX), superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) activities ferric reducing antioxidant power (FRAP) capacity and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging capacity of different road vibration treatments and control treatment without vibration during storage at room temperature (23 ℃) for 28 days were compared. The results showed that the total phenolics and VC contents, APX, SOD, CAT and POD activities, FRAP and DPPH radical scavenging capacity of Hami melon under different grades of road vibration were lower than those of the control group at day 28 of storage, and SR and TR caused significantly higher respiration rate and greater damage to the antioxidant system of Hami melon than expressway and AR (P < 0.05), which indicated that vibration can increase respiration rate and reduce the activity of various enzymes and antioxidants in the antioxidant protection system during the storage of cantaloupe, accelerating cell membrane lipid oxidation, destroying the integrity of the cell membrane, and finally accelerating the senescence and spoilage of melons. The effect of transportation vibration on SR and TR was more obvious.