碱性电解水耦合酶提取茶渣蛋白工艺优化

茶渣蛋白具有很好的抗氧化活性,本文以茶渣为原料,研究了碱性电解水耦合酶提取茶渣蛋白的工艺。在单因素实验的基础上,采用正交实验设计优化了碱性电解水提取茶渣蛋白工艺,并优化碱性蛋白酶耦合碱性电解水提取茶渣蛋白工艺。正交结果表明,碱性电解水提取的最佳条件为:pH12.5,提取温度120 ℃,液固比40:1 (mL/g),提取时间40 min,茶渣蛋白提取率为48.3%。耦合酶提取结果表明:碱性蛋白酶添加量为2500 IU/g茶渣,酶解pH为9,酶解时间32 h,茶渣蛋白提取率提高至83%,较单一碱性电解水提取提高34.7%,同时蛋白质总抗氧化能力较高。本研究结果为茶渣的综合利用及蛋白资源的有效开发提供了一定的理论依据。

Optimization of Extraction Process of Protein from Exhausted Tea by Alkaline Electrolyzed-Reducing Water Synergized with Protease

Protein extracted from exhausted tea possessed great antioxidant activity. In this study,alkaline electrolyzed-reducing water synergized with protease was adopted to extract protein from exhausted tea. Based on the single factor experiments,orthogonal test was employed to optimize the extraction conditions of protein from exhausted tea,and an alkaline protease was further selected to improve the protein extraction rate. Orthogonal test results showed that the optimal extract conditions were as follows:pH12.5,extraction temperature 120 ℃,extraction time 40 min and 40∶1 of liquid-solid ratio(mL/g),and the extraction rate of protein yield reached to 48.3% under the optimal conditions. The extraction parameters of protein in the exhausted tea by protease were further optimized,the results showed that the yield of protein in exhausted tea was 83% under the extraction conditions:pH9,hydrolysis time 32 h,enzyme amount 2500 IU/g exhausted tea. Compared with the former,extraction rate of protein in exhausted tea increased significantly with the synergistic action of alkaline protease by 34.7%. Besides,the protein possessed high total antioxidant capacity. This study provided a theoretical basis for the comprehensive utilization of exhausted tea and the effective development of new protein resources.