从蚕砂中制备脱镁叶绿酸的工艺研究

目的 :优化以蚕砂为原料制备脱镁叶绿酸工艺条件 ,提高产出率。方法 :以叶绿素提取率为指标 ,对脱镁叶绿酸制备的乙醇法和丙酮法工艺进行比较 ,用正交试验法对丙酮法的工艺条件进行优化。结果 :脱镁叶绿酸的最佳制备条件为蚕砂粉碎度60目 ,加1/10倍药材量的水软化5h以上 ,加3倍药材量的溶媒 ,提取温度70℃ ,回流时间60min ,提取次数2次 ,酸化时 pH调至2 5。结论 :软化水用量、丙酮量对叶绿素提取率影响较大 ,经优化后可提高丙酮法制备脱镁叶绿酸的产出率4 6倍

Study on the Preparation Technic of Pheophrobide From Silkworm Excrement

OBJECTIVE:To optimize the factors of preparing pheophrobide from silkworm excrement and raise the yield of pheophrobide.METHODS:Based on the extraction rate of chlorophyll,acetone method was compared with alcohol method and the preparing factors of acetone method was optimized by orthogonal design.RESULTS:The best conditions for pheophrobide preparation included grinding silkworm excrement into powder with particle size all below 250μ m,softening the powder more then 5h with water 1/10 the volume of powder,extracting the powder with acetone three times the volume of powder at 70℃ for two times 60min each,regulating pH of the liquid to 2.5 with HCl solution.CONCLUSION:The water and acetone volume was more important than other factors,the yield of pheophrobide was raised 3.6 times after optimization