| 双鞭甲藻液体菌种培养条件优化 |
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| 引用本文： | 李正鹏,尚耘. 双鞭甲藻液体菌种培养条件优化[J]. 食品工业科技, 2010, 0(3) |
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| 作者姓名： | 李正鹏 尚耘 |
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| 作者单位： | 1. 安徽科技学院生命科学学院,安徽,凤阳,233100
2. 武汉烯王生物工程有限公司,湖北,武汉,430073 |
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| 摘 要： | 以微藻生物量为指标,对产DHA藻类液体培养条件进行优化。在正交实验和单因素实验的基础上,确定发酵培养条件为:葡萄糖40g/L、谷氨酸钠10g/L、蛋白胨2g/L,人工海水500mL/L,转速160r/min,初始pH6.5,接种量10%,装液量100mL/250mL,28℃培养5d,微藻生物量达1.63g/100mL。
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| 关 键 词： | 双鞭甲藻 液体菌种 培养条件 |
Optimization of liquid strain fermentation conditions for crypthecodinium cohnii |
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| LI Zheng-peng,SHANG Yun. Optimization of liquid strain fermentation conditions for crypthecodinium cohnii[J]. Science and Technology of Food Industry, 2010, 0(3) |
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| Authors: | LI Zheng-peng SHANG Yun |
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| Affiliation: | LI Zheng-peng1,SHANG Yun2 (1.School of Life Science,Anhui Science , Technology University,Fengyang 233100,China,2.Wuhan Alking Bioengineering Co.,Ltd.,Wuhan 430073,China) |
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| Abstract: | Optimization of liquid culture conditions was carried out with biomass as target.According to the orthogonal design,the results showed that the fermentation condition was glucose 40g/L,sodium glutamate 10g/L,peptone 2g/L,sea water 500mL/L,initial pH 6.5, inoculation rate 10% ,volume 100mL/250mL.The biornass reached 1.63 g/100mL, after cultured 5 d, at the temperature of 28"C. |
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| Keywords: | crypthecodinium cohnii liquid strain fermentation condition |
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