| 聚结过滤法测定β-榄香烯固体脂质纳米粒的包封率 |
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| 引用本文： | 王艳芝,郑甲信,胡会国,邓意辉,毕殿洲,孙伟.聚结过滤法测定β-榄香烯固体脂质纳米粒的包封率[J].沈阳药科大学学报,2007,24(5):271-274. |
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| 作者姓名： | 王艳芝 郑甲信 胡会国 邓意辉 毕殿洲 孙伟 |
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| 作者单位： | 1. 郑州大学药学院,河南,郑州,450001
2. 沈阳药科大学药学院,辽宁,沈阳,110016 |
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| 摘 要： | 目的建立聚结过滤法测定β-榄香烯固体脂质纳米粒(SLN)的包封率。方法先以体积分数为36%的乙醇水溶液溶解SLN制剂中的游离药物,然后用氯化钠水溶液聚结SLN并过微孔滤膜,以HPLC法测定滤液中β-榄香烯含量,计算包封率。结果该方法可将游离药物与SLN分开,平均回收率为97.4%,测得的β-榄香烯SLN的包封率为96.0%。结论使用聚结过滤法可准确、快速地分离出游离β-榄香烯,可用于该制剂包封率的测定。
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| 关 键 词： | β-榄香烯 固体脂质纳米粒 包封率 聚结过滤法 |
| 文章编号： | 1006-2858(2007)05-0271-04 |
| 修稿时间： | 2006-06-26 |
Determination of the entrapment efficiency of β-elemene did lipid nanoparticles by method coagulation-filtration |
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| WANG Yan-zhi,ZHENG Jia-xin,HU Hui-guo,DENG Yi-hui,BI Dian-zhou,SUN Wei.Determination of the entrapment efficiency of β-elemene did lipid nanoparticles by method coagulation-filtration[J].Journal of Shenyang Pharmaceutical University,2007,24(5):271-274. |
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| Authors: | WANG Yan-zhi ZHENG Jia-xin HU Hui-guo DENG Yi-hui BI Dian-zhou SUN Wei |
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| Affiliation: | 1. School of Pharmacy, Zhengzhou University, Zhengzhou 450001, China; 2. School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China |
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| Abstract: | Objective To determine the entrapment efficiency of β-elemene solid lipid nanoparticle(SLN) by method of coagulation-filtration.Methods After consequently addition of agueous alcohol solution and agueous sodium chloride to the SLN,the dispersion was filtered and the solution containing free drug was obtained,content of β-elemene was determined by HPLC.Results Under the established condition,free β-elemene could be well separated from the preparation.The average recovery of β-elemene was 97.4%.The entrapment efficiency of β-elemene SLN was(96.0±0.41)%.Conclusions The method is quick and accurate to obtain free β-elemene for the determination of entrapment efficiency of β-elemene SLN. |
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| Keywords: | β-elemene solid lipid nanoparticle(SLN) entrapment efficiency coagulation-filtration method |
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