响应面试验优化超声波辅助提取圆叶葡萄鞣花酸和总酚工艺

以圆叶葡萄Noble皮和籽为原料，使用丙酮提取溶剂（丙酮-水-盐酸体积比70∶29∶1），采用超声波辅助提取圆叶葡萄中以鞣花酸为主的多酚类化合物。在单因素试验的基础上，利用响应面试验优化圆叶葡萄皮中鞣花酸和总酚提取工艺，建立数学回归模型，并分析双因素间的交互作用。结果表明，影响圆叶葡萄皮中鞣花酸和总酚含量的显著因素为料液比、提取时间和超声功率，得到的最佳提取工艺条件为料液比1∶30（g/mL）、提取时间28 min、超声功率616 W，此条件下鞣花酸和总酚含量分别为616.21 μg/g和15.06 mg/g（以没食子酸当量计，鲜质量）。用响应面试验对圆叶葡萄中鞣花酸和总酚提取进行优化，可得到最佳工艺条件，验证结果与理论预测拟合度高，可为实际生产提供理论依据。

Optimization of Ultrasound-Assisted Extraction of Ellagic Acid and Total Phenols from Muscadine (Vitis rotundifolia) by Response Surface Methodology

The extraction conditions of ellagic acid and total phenols from the skin and seeds of the Noble muscadine
using acetone/water/hydrochloric acid (70:29:1, V/V) by ultrasonic-assisted extraction (UAE) were optimized employing a
combination of single-factor experiments and response surface methodology through mathematical modeling. The analysis
of two-factor interactions was carried out. The results indicated the ratio of solid to liquid, extraction time and ultrasonic
power to be the most significant factors affecting the extraction efficiencies of ellagic acid and total phenols. The optimum
extraction conditions for ellagic acid and total phenols from muscadine skin were determined as follows: ratio of solid to
liquid, 1:30; extraction time, 28 min; and ultrasonic power, 616 W. The predicted contents of ellagic acid and total phenols
under these conditions were 616.21 μg/g and 15.06 mg/g (using gallic acid equivalent, fresh weight), respectively, agreeing
with the experimental values. In conclusion, UAE can be applied to the extraction of ellagic acid.