硒协同中药多糖对白血病细胞株P388体外作用的实验研究

目的:研究香菇多糖协同硒酸酯多糖对白血病细胞的抑制作用,探讨其相关机理。方法:MTT法检测香菇多糖协同硒酸酯多糖对白血病细胞增殖的抑制作用,流式细胞术检测细胞凋亡,免疫细胞化学检测相关基因Fas和FasL表达水平的改变。结果:香菇多糖和硒酸酯多糖可抑制白血病细胞增殖,硒酸酯多糖主要通过上调Fas和FasL促进凋亡发挥肿瘤抑制作用,香菇多糖主要通过上调Fas促进细胞凋亡,FasL表达没有变化。结论:二者存在不同抑癌机理。香菇多糖协同硒酸酯多糖可显著提高白血病抑制效应。

Experimental Study on Effect of Chinese Tradtional Medicine Polysaccharides in Combination with Selenium on P388 Leukemia Mice

Objective:To study on the inhibitory effect of lentinan in combination with kappa-selenocarrageenan on leukemia and its molecular mechanism of antileukemia.Methods: The inhibitory effect of lentinan in combination with kappa-selenocarrageenan on leukemia cells was detected by MTT methods.The extent of cell apoptosis was measured by flow cytometry.The expressive levels of Fas and FasL were detected by immunochemistry assay.Results: Lentinan in combination with kappa-selenocarrageenan can inhibit the proliferation of leukemia cells.Kappa-selenocarrageenan plays tumor-suppressing roles through upregulating the expression of Fas and FasL and improving apoptosis,and lentinan by upregulating the expression of Fas but no effect on FasL.Conclusion:Lentinan and kappa-selenocarrageenan have different tumor-suppressing mechanisms.Lentinan in combination with kappa-selenocarrageenan can markedly improve the inhibiting effect on leukemia.