立论于肝脾同治研究逍遥散对老年性痴呆模型鼠行为学及神经可塑性的影响

目的:立论于肝脾同治研究逍遥散对老年性痴呆模型鼠行为学及神经可塑性生长相关蛋白(GAP-43)和突触素(SYN)的影响。方法:72只健康SPF级雄性大鼠,根据随机数子表法随机分为3组,假手术组、痴呆组和逍遥散组,每组各24只。痴呆组和逍遥散组进行老年痴呆AD大鼠的模型制备,假手术组:常规饲料饮水SPF级饲养;痴呆组:在空白组的基础上,给予等量0.2 m L/10 g无菌生理盐水灌胃;逍遥散组:在空白组的基础上,逍遥散水煎液,给予0.2 m L/10 g的逍遥散水煎液灌胃。1次/d,疗程6周。采用Y型迷宫进行大鼠的学习记忆行为检测,透射电子显微镜(TEM)观察海马CA1区突触结构,免疫组化检测海马CA1区生长相关蛋白(GAP-43)的表达情况,蛋白质印迹法(Western Blot)检测突触素(SYN)的表达情况。结果:1)与假手术组比较,痴呆组和逍遥散组中EN均增加(P0.05),CN均减少(P0.05);其中逍遥散组EN低于痴呆组(P0.05),CN则高于痴呆组(P0.05)。2)TEM下观察假手术组突触结构和形态正常,逍遥散组大鼠海马CA1区突触结构和形态均较痴呆组规则(P0.05)。3)与假手术比较,痴呆组和逍遥散组GAP-43和SYN表达均下调(P0.05),而逍遥散组中GAP-43和SYN的表达则高于痴呆组(P0.05)。结论:逍遥散(肝脾同治)有效改善老年性痴呆模型鼠的学习记忆行为学和突触结构形态,其机制可能与上调了生长相关蛋白(GAP-43)免疫组化8和突触素(SYN)神经可塑性相关蛋白有关。

Effect of Xiaoyao Powder on Praxiology and Neural Plasticity in Rats with Alzheimer's Diseases from Simultaneous Treatment in Liver and Spleen Theory

To study the effect of Xiaoyao Powder on praxiology and neural plasticity of growth protein (GAP-43) and synaptophysin (SYN) in rats with alzheimer''s diseases from simultaneous treatment in liver and spleen theory. Methods: Seventy-two healthy male SPF rats were randomly divided into 3 groups: sham operation group, dementia group and Xiaoyao Powder group, with 24 rats in each group. Rats in the dementia group and Xiaoyao Powder group had operation for senile dementia in AD model. Sham operation group received conventional feeding of grade SPF; dementia group was additionally treated with 0.2 mL/10 g sterile saline, while Xiaoyao Powder group received Xiaoyao Powder based on the blank group treatment and was given by 0.2 mL/10 g decoction lavage, once a day for 6 weeks. Y maze was applied for the study of learing and memorial behavior, and synaptic structure in hippocampal CA1 region was detected by transmission electron microscopy (TEM), immunohistochemistry was used to detect the expression of hippocampus CA1 growth protein (GAP-43), and Western blot (Western Blot) was applied to detect the expression of synaptophysin (SYN). Results: 1) Compared with the sham operation group, EN of the dementia group and Xiaoyao Powder group was increased (P<0.05) and CN decreased (P<0.05). EN in the Xiaoyao Powder group was lower than that of dementia group (P<0.05) and CN was higher than that in senile dementia group (P<0.05). 2) The structure and morphology of the synapses were observed in the sham operated group. The structure and morphology of CA1 in the hippocampus of the Xiaoyao Powder group were significantly lower than those in the dementia group (P<0.05). 3) Compared with sham operation group, the expression of GAP-43 and SYN in the dementia group and the Xiaoyao Powder group were all down-regulated (P<0.05), while the expression of GAP-43 and SYN in Xiaoyao Powder group was higher than that in dementia group (P<0.05). Conclusion: Xiaoyao Powder (from simultaneous treatment for Liver and spleen theory) may effectively improve the learning and memorial behavior of senile dementia model rats and its synaptic structure form. Its mechanism may be related to the up-regulation of growth associated protein (GAP-43) immunohistochemistry 8 and synaptophysin (SYN) on neural plasticity associated protein.