Development of an amphiphilic matrix for immobilization of <Emphasis Type="Italic">Candida antartica</Emphasis> lipase B for biodiesel production |
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| Authors: | Ko Woon Lee Kyoungseon Min Kyungmoon Park Young Je Yoo |
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| Affiliation: | (1) Department of Chemical and Biological Engineering, Korea University, 1, Anam-dong, Sungbuk-ku, Seoul, 136-701, Korea;(2) Department of Chemical Engineering, Kwangwoon University, 447-1, Wolgye-Dong, Nowon-Gu, Seoul, 139-701, Korea;(3) Department of Chemical Engineering, Hankyong National University, 67 Sukjung-dong, Ansung-city, Kyonggi-do, 456-749, Korea; |
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| Abstract: | Biodiesel has been greatly interested as an alternative fuel and is produced by a transesterification reaction of oil with
alcohol. Recently, microbial lipases have been used for biodiesel production. Among the microbial lipase, immobilized Candida antartica lipase B (CALB) is the most widely used. However, CALB is unstable and shows low catalytic efficiency in the reaction media
because the reaction media contains a high concentration of methanol and the lipase is also inhibited by the by-product glycerol.
In this study, to overcome these limitations, we developed an amphiphilic matrix to immobilize CALB. The immobilized lipase
in an amphiphilic matrix with 80% ethyltrimethoxysilane (ETMS) in tetramethoxysilane (TMOS) and pretreated with oil showed
the highest specific activity and biodiesel conversion ratio; about 90% biodiesel conversion in 24 h at an initial molar ratio
of 1: 1 (oil: methanol) with stepwise methanol feeding in order to adjust the net molar ratio to be 1: 3. |
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