| 正交法优化保加利亚乳杆菌冻干保护剂 |
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| 引用本文： | 陈合,李串娜,舒国伟,罗茜.正交法优化保加利亚乳杆菌冻干保护剂[J].西北轻工业学院学报,2013(2):89-92. |
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| 作者姓名： | 陈合 李串娜 舒国伟 罗茜 |
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| 作者单位： | 陕西科技大学生命科学与工程学院 |
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| 基金项目： | 陕西省科技厅工业攻关项目(2012K08-17) |
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| 摘 要： | 通过单因素实验和正交试验设计，研究了乳糖、脱脂乳粉、蔗糖和抗坏血酸钠等4种物质对保加利亚乳杆菌的冻干存活率及菌粉活菌数的影响，确定了适合保加利亚乳杆菌的最佳冻干保护剂配方．结果表明，4种物质的保护效果依次为：蔗糖〉脱脂乳粉〉抗坏血酸钠〉乳糖；最佳保护剂的配方为：30％蔗糖＋209／6脱脂乳粉＋4．0％抗坏血酸钠＋20％乳糖．加入冻干保护剂后，保加利亚乳杆菌冻干后存活率可达94％，菌粉的活菌数达1．926×10^11 cfu／g．
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| 关 键 词： | 保加利亚乳杆菌 冷冻干燥 保护剂 正交优化 |
Optimization of freeze-drying cryoprotectants for Lactobacillus bulgaricus by orthogonal experiment |
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| CHEN He,LI Chuan-na,SHU Guo-wei,LUO Qian.Optimization of freeze-drying cryoprotectants for Lactobacillus bulgaricus by orthogonal experiment[J].Journal of Northwest University of Light Industry,2013(2):89-92. |
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| Authors: | CHEN He LI Chuan-na SHU Guo-wei LUO Qian |
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| Affiliation: | (College of Life Science and Engineering,Shaanxi University of Science & Technology,Xi′an 710021,China) |
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| Abstract: | The effect of Lactose, skim milk powder, sucrose and sodium ascorbate on survival and viable cells of freeze-dried Lactobacillus bulgaricus was studied by single factor and or- thogonal experiments. The results were as follows： the order of the influencing factors was sucrose〉skimmed milk powder〉sodium ascorbate〉Lactose. The best cryoprotectants in- gredient was：30%sucrose＋20% skimmed milk powder＋4.0Gsodium ascorbate＋20%Lac- tose, the survival rate and viable counts of Lactobacillus bulgaricus during freeze-dried is up to 94% and 1. 926×10^11 cfu/g when were added to the cryoprotectants. |
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| Keywords: | Lactobacillus bulgaricus freeze-drying cryoprotectant orthogonal optimization |
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