海洋低温酶酶解贻贝的动力学研究

用海洋低温碱性蛋白酶在温度30℃、pH为10.00条件下,对贻贝肉蛋白进行酶解。用相关的动力学模型推导公式与实验相结合,研究其酶解机制。结果表明:在反应过程中存在着酶失活现象,以及底物存在着促进反应进行和抑制酶活性的双重作用;得到的水解度动力学方程及水解速率方程,结果表明酶催化水解速率随水解进程呈指数递减;失活常数k_d=13.989 min^-1;动力学模型计算值与实验值平均相对偏差为5.80%,此结果说明该动力学模型与实验测量结果有很高的吻合性。

Kinetic model for the enzymolysis of mussel by marine microtherm alkaline protease

Enzymatic hydrolysis of mussel meat protein was studied by marine microtherm alkaline protease under the conditions of temperature 30℃and pH 10.00.The enzymolysis mechanism was researched with the method of combining related dynamics model derivation formula with experiments.Results showed that:the phenomenon of enzymes inactivation existed during the reaction,and substrates existed double actions of promoting reaction and restraining enzymes activity.The dynamics equation of hydrolysis degree and hydrolysis rate equation indicated that enzyme hydrolysis speed decreased exponentially with hydrolysis process.Inactivation constant k_d was 13.989 min^-1. The average relative deviation of dynamics model calculation values with experimental values was 5.80%,this result indicated that dynamic model and the experimental results had high coincidence.