Studies on Nucleotide Chemistry 15. Synthesis of Oligodeoxynucleotides Using Amidine Protected Nucleosides |
| |
| Authors: | Marvin H Caruthers Lincoln J Mcbride Laurent P Bracco John W Dubendorff |
| |
| Affiliation: | Department of Chemistry , University of Colorado , Boulder, Colorado, USA |
| |
| Abstract: | Abstract An in situ method is described for synthesizing DNA which incorporates a new series of amidine protected deoxy-nucleosides and bis-dialkylaminophosphines as phosphitylating agents. These procedures were used to synthesize d(GGGAATTCCC) which was digested by EcoRI. |
| |
| Keywords: | |
|
|
