| 纳米二氧化铈化学发光抑制法测定抗坏血酸 |
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| 引用本文： | 石文兵,贺 薇,万帮江.纳米二氧化铈化学发光抑制法测定抗坏血酸[J].食品科学,2015,36(24):112-115. |
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| 作者姓名： | 石文兵 贺 薇 万帮江 |
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| 作者单位： | 无机特种功能材料重庆市重点实验室，长江师范学院化学化工学院，重庆 408100 |
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| 基金项目： | 国家自然科学基金面上项目（21275021）；重庆市教委科学研究项目（KJ1401201；KJ1401220）；
长江师范学院青年科研人才成长支持计划项目 |
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| 摘 要： | 基于抗坏血酸抑制纳米二氧化铈-过氧化氢-鲁米诺体系的发光强度，建立流动注射化学发光法检测抗坏血酸含量的新方法。考察过氧化氢浓度、鲁米诺浓度、纳米二氧化铈质量浓度及pH值对发光强度的影响。在优化条件下，该方法对抗坏血酸检测的线性范围为0.001～1.0 mg/mL（r = 0.983 1），检出限（3 σ）为0.2 ng/mL，对质量浓度为1.0 mg/mL的抗坏血酸进行11 次平行测定，相对标准偏差为2.9%。该法已成功用于水果、果汁和蔬菜中抗坏血酸含量的测定，方法简单、灵敏、快速。
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| 关 键 词： | 纳米二氧化铈 化学发光 抗坏血酸 |
Determination of Ascorbic Acid Based on its Inhibition on Nano-Ceria Chemiluminescence |
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| SHI Wenbing,HE Wei,WANG Bangjiang.Determination of Ascorbic Acid Based on its Inhibition on Nano-Ceria Chemiluminescence[J].Food Science,2015,36(24):112-115. |
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| Authors: | SHI Wenbing HE Wei WANG Bangjiang |
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| Affiliation: | Chongqing Key Laboratory of Inorganic Special Functional Materials, College of Chemistry and Chemical Engineering,
Yangtze Normal University, Chongqing 408100, China |
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| Abstract: | A new flow injection chemiluminescence (CL) method based on the inhibition of ascorbic acid on nano-ceria-
H2O2-luminol CL system has been developed for the determination of ascorbic acid (AA). The effects of concentrations of
H2O2, luminol and nano-ceria, and pH on CL intensity were investigated respectively. Under the optimum conditions, the
linear range of the method was 0.001–1.0 mg/mL (r = 0.983 1), and the detection limit (3σ) was 0.2 ng/mL. The relative
standard deviation (RSD) for 11 repetitive determinations of 1.0 mg/mL AA was 2.9%. The method is simple, sensitive and
fast and has been successfully applied to determine ascorbic acid in fruits, juices and vegetables. |
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| Keywords: | nano-ceria chemiluminescence ascorbic acid |
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