rhEPO对大鼠肝脏缺血再灌注损伤的保护作用

目的:探讨rhEPO对大鼠肝脏缺血再灌注损伤中的保护作用及其处理时间。方法:健康Wistar大鼠36只,随机分为对照组A、缺血前30 min预处理组B和缺血后5 min后处理组C 3组,每组12只。A组只给予夹闭肝蒂,B、C组均于皮下注射rhEPO 1 000 U/kg,三组均夹闭肝蒂45 min后恢复再灌注2 h,取血清及肝组织行AST、ALT、TNF-α、IL-1β检测及HE染色、NF-κβ的活化程度及计算肝组织凋亡指数(AI)。结果:与A组比较,B、C组肝细胞索排列较好,细胞坏死程度不明显。AST、ALT和TNF-α、IL-1β及NF-κB活化程度、AI在A组和B、C组之间均有显著差异(P<0.05),B和C组之间(除IL-1β外)也具有统计学意义(P<0.05)。结论:rhEPO对缺血再灌注损伤的肝脏具有保护作用且预处理较后处理效果好。

Protection of rhEPO on hepatic ischemic/reperfusion injury in rats

Objectives:To explore the protective effect of rhEPO on hepatic ischemia reperfusion injury in rats and treatment time.Methods:Thirty-six wistar rats were randomly divided into three groups,the control group A,the rhEPO pretreatment group B and postprocessing group C,n=12 respectively.Liver pedicles were occlused in group,rats were injected rhEPO 1 000 U/kg into subcutaneously 30 minutes before ischemia in B group or 5 minutes after in C group A.After 45 minutes ischemia,then followed reperfusion.Serum aspartic acid aminotransferase(AST)and alanine aminotransferase(ALT),tumor necrosis factor α(TNF-α) and interleukin 1 β(IL-1β)were measured and liver histo-pathology was observed by HE staining.Nuclear factor κB(NF-κB) was detected by immunohistochemistry and apoptotic index was calculated by TUNEL.Results:Compared to group A,the arrargement of liver cell-line is relatively better and the cells were damaged insignificantly in group B and C.The serum concentration of AST,ALT,TNF-α,IL-1β,NF-κB and AI in group A have difference significantly(P<0.05),and group B and C had statistical significance(except IL-1β).Conclusion:RhEPO protects the liver from ischemia reperfusion injury in rats and pretreatment is better than postprocessing.