大黄素对脓毒症大鼠保护作用的实验研究

目的探讨大黄素对于脓毒症大鼠的保护作用。方法将健康大鼠随机分为三组,A组：假手术组;B组：盲肠结扎穿刺（CLP）组;C组：CLP大黄素治疗组。造模72h后分别测定各组血浆肿瘤坏死因子-α（TNF-α）、血浆和腹腔白蛋白浓度、肠系膜微静脉流速等并观察肠粘膜损伤程度。结果CLP后大鼠血浆TNF-α浓度、白蛋白渗出率明显升高,而B组升高较C组明显（P〈0.01）,肠粘膜损伤评分变化也有相同趋势（P〈0.017）;肠系膜微静脉流速减慢,血浆白蛋白浓度降低,而B组下降较C组明显（P〈0.05）。结论大黄素能减轻CLP所致脓毒症大鼠的炎症反应和肠粘膜的损伤。

Experimental study on protective effect of trioxymethylanthraquinone to rat sepsis

Objective To investigate the protective effect of trioxymethylanthraquinone to rat sepsis. Methods Healthy rats were divided into 3 groups, group A as control group; group B as caecal ligation plus puncture （CLP） group and group C as trioxymethylanthraquinone group. Plasma concentrations of TNF - α, mesenterium microcirculation flow rate, plasma and peritoneal concentrations of albumin were measured, pathological changes in intestinal mucosa were observed 72h after operation. Results TNF- α and ratio of plasma albumin versus peritoneal albumin were notably increased after CLP, and it was lower in group C than in group B（ P 〈 0.01 ）. Less damage in intestinal mucesa was observed in group C than in group B（ P 〈 0. 017）. Mesenterium micrecirculation flow rate and plasma albumin were decreased after CLP, and they were higher in group C than in group B （ P 〈 0.05 ）. Conclusions trioxymethylanthraquinone could reduce the inflammatory response and extenuate the damage to intestinal mucosa in rat sepsis.