金匮肾气丸对肾阳虚模型小鼠肺和气道组织GR、β-防御素-2表达的调控作用

目的:探讨金匮肾气丸对肾阳虚模型小鼠气道和肺组织中糖皮质激素受体(GR)、β-防御素-2(BD-2)及其mRNA表达的影响和意义。方法:用腺嘌呤灌胃复制小鼠肾阳虚模型,并在腺嘌呤灌胃的同时予金匮肾气丸灌胃干预,通过免疫组化法(SP)测定肺及气道组织GR和BD-2的表达水平,原位杂交(ISH)法对GR和BD-2 mRNA表达进行定位定量分析。结果:与正常对照组比较,肾阳虚组肺和气道组织GR及其mRNA表达下调(P<0.05),BD-2及其mRNA表达上调(P<0.05),肺组织呈炎症改变;与肾阳虚组比较,金匮肾气丸组GR及其mRNA表达上调(P<0.05),BD-2及其mRNA无统计学差异(P>0.05),但肺组织炎症程度减轻。结论:金匮肾气丸可通过上调肾阳虚小鼠肺和气道组织GR及其mRNA表达而抑制肺组织炎症,这可能是金匮肾气丸纠正肾阳虚证的分子机制之一。

Regulative effects of Jingui Shenqi bolus on glucocorticoid-receptor,β-defensin-2 expressions in the airway epithelial cells and lung tissues of mice with renal-yang asthenia

Objectives:To investigate the regulative effects of the Jingui Shenqi bolus(JGSQB) on glucocorticoid receptor(GR),β-defensin-2(BD-2) and their mRNA expressions in the airway epithelial cells and lung tissues of mice with renal-yang asthenia(RYA).Methods:The RYA mice were induced by giving adenine intragestrically,as well as adding the JGSQB in the JGSQB group.The GR,BD-2 and their mRNA expressions were detected respectively by immunohistochemistry and in situ hybridization.Results:Compared with the NS group,the GR and mRNA expressions were down-regulated(P<0.05),and the BD-2 and mRNA were up-regulated(P<0.05) in the airway epithelial cells and lung tissues with inflammation change in the RYA mice.Compared with the RYA group,the GR and its mRNA expression were up-regulated with inflammation decrease(P<0.05),and no effects on the BD-2 and its mRNA(P> 0.05)in the airway epithelial cells and lung tissues in the JGSQB mice.Conclusion:JGSQB could up-regulate GR and mRNA expressions and inhibit the inflammation,these may be the molecular mechanism of the JGSQB to treat RYA syndrome.