| AB-8树脂纯化柿叶总黄酮的工艺研究 |
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| 引用本文： | 马燕燕,鲁晓翔.AB-8树脂纯化柿叶总黄酮的工艺研究[J].食品工业科技,2011(1):243-245,308. |
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| 作者姓名： | 马燕燕 鲁晓翔 |
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| 作者单位： | 天津商业大学生物技术与食品科学学院,天津市食品生物技术重点实验室,天津,300134 |
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| 摘 要： | 目的:筛选出分离纯化柿叶总黄酮的最佳树脂,并对影响分离纯化的因素进行研究,得到优化的纯化条件。方法:选用AB-8、ADS-17和D3520三种型号大孔吸附树脂,采用动态吸附-解吸方法,利用分光光度法测定黄酮含量,研究了不同的大孔吸附树脂及其不同的工艺条件对柿叶黄酮分离纯化的影响。结果:实验表明AB-8树脂的分离效果最好,其最佳工艺为:上柱液pH6,上柱液流速2BV/h,样液浓度为3mg/mL,70%乙醇为洗脱液,洗脱液流速控制在2BV/h,洗脱液用量为3BV。在此条件纯化后,柿叶黄酮提取物中黄酮含量由9%提高到34%。结论:AB-8大孔树脂可以较好地分离纯化柿叶黄酮。
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| 关 键 词： | 柿叶 总黄酮 大孔吸附树脂 分离纯化 |
Separation and purification process of total flavonoids from persimmon leaf with AB-8 resin |
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| MA Yan-yan,LU Xiao-xiang.Separation and purification process of total flavonoids from persimmon leaf with AB-8 resin[J].Science and Technology of Food Industry,2011(1):243-245,308. |
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| Authors: | MA Yan-yan LU Xiao-xiang |
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| Affiliation: | MA Yan-yan,LU Xiao-xiang(Biotechnology and Food Science College,Tianjin University of Commerce,Tianjin Key Laboratory Food of Biotechnology,Tianjin 300134,China) |
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| Abstract: | Objective:To choose the optimum resin for separation and purification of persimmon leaf flavonoids and analyze influenceable factors of separation and purification process. Methods:Three macroporous resins including AB-8,ADS-17and D3520 were used for separation of total flavonoids from persimmon leaf. During the adsorption and desorption process,with UV to assay total flavonoids,the different macroporous resins and their capacities were studied. Results:AB-8 resin possesses had much higher adsorption and de... |
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| Keywords: | persimmon leaf flavomoids macroporous resin separation and purification |
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