| 实时荧光PCR法检测食物中榛子过敏原成分 |
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| 引用本文： | 孙敏,梁君妮,徐彪,高宏伟,林超,刘彩霞.实时荧光PCR法检测食物中榛子过敏原成分[J].食品工业科技,2011(1):293-295,299. |
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| 作者姓名： | 孙敏 梁君妮 徐彪 高宏伟 林超 刘彩霞 |
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| 作者单位： | 1. 山东出入境检验检疫局,山东,青岛,266002
2. 烟台出入境检验检疫局,山东,烟台,264000 |
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| 摘 要： | 榛子是最常见的食品过敏原之一,选择针对榛子不同基因的引物和探针,进行验证、比对和条件优化,建立榛子过敏原的实时荧光PCR检测方法。经实验比较,针对榛子热休克蛋白设计合成的引物和探针具有相对较高的灵敏性,检测限可达0.11ng DNA,而且和常见的食物种类无交叉反应,适于榛子过敏原的检测。该方法的建立,对于加强食品过敏原的标识管理,保护消费者利益、促进进出口贸易具有重要意义。
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| 关 键 词： | 实时荧光PCR 过敏原 检测 |
Detection of hazelnut allergens by real time PCR assay |
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| SUN Min,LIANG Jun-ni,XU Biao,GAO Hong-wei,LIN Chao,LIU Cai-xia.Detection of hazelnut allergens by real time PCR assay[J].Science and Technology of Food Industry,2011(1):293-295,299. |
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| Authors: | SUN Min LIANG Jun-ni XU Biao GAO Hong-wei LIN Chao LIU Cai-xia |
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| Affiliation: | SUN Min1,LIANG Jun-ni2,XU Biao1,GAO Hong-wei1,LIN Chao1,LIU Cai-xia1(1.Shandong Entry-Exit Inspection and Quarantine Bureau,Qingdao 266002,China,2.Yantai Entry-Exit Inspection and Quarantine Bureau,Yantai 264000,China) |
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| Abstract: | Hazelnut is one of the most common allergens. After experimenting on primers and probes specific to different genes of hazelnut and optimizing experimental parameters,a real time PCR assay for detecting hazelnut allergen was established. The primers and probes targeting hsp1 gene were proved to be preferable,which had a sensitivity up to 0.11ng purified DNA and had no cross-reactions with other species. This assay may provide a potential tool for food allergens supervision,which is of importance in protecti... |
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| Keywords: | real time PCR allergen detection |
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