超高效液相色谱法同时测定饮料中5种人工合成色素

目的：建立超高效液相色谱同时测定饮料中5种人工合成色素(柠檬黄、苋菜红、胭脂红、日落黄、亮蓝)的检测方法。方法：样品采用聚酰胺吸附法提取色素，通过Waters ACQUITY UPLCTM BEH C18反相色谱柱分离，以甲醇-0.02mol/L乙酸铵缓冲溶液为流动相进行梯度洗脱，于254nm和629nm波长处检测。结果：5种人工合成色素在5.5min内完成分离，3～80mg/L范围内呈良好线性关系(r＞0.9995)，检出限为0.03～0.10mg/L，平均回收率为97.4%～107.3%，RSD为0.22%～3.20%。结论：该方法简便、快速、分离效果好、灵敏度高，适用于饮料中人工合成色素的测定。

Determination of Five Synthetic Pigments in Beverage by Ultra Performance Liquid Chromatography

Objective:To develop an ultra performance liquid chromatography (UPLC) method for the determination of five synthetic pigments such as tatrazine,amaranth,carmine,sunset yellow and brilliant blue in beverage. Methods:Synthetic pigments in beverage were extracted by polyamide adsorption method,separated on a Waters ACQUITY UPLCTM BEH C18 column by gradient elution using a mobile phase made up of methanol and 0.02 mol/L ammonium acetate,and detected at 254 nm and 629 nm. Results:The five synthetic pigments wer...