| 苦荞萌发物中生物活性黄酮对人乳腺癌细胞增殖的抑制作用 |
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| 引用本文： | 周小理,王青,杨延利,李宗杰,唐文.苦荞萌发物中生物活性黄酮对人乳腺癌细胞增殖的抑制作用[J].食品科学,2011,32(1):225-228. |
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| 作者姓名： | 周小理 王青 杨延利 李宗杰 唐文 |
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| 作者单位： | 1.上海应用技术学院香料香精技术与工程学院 2.上海海洋大学食品学院 3.上海师范大学生命与环境科学学院 |
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| 基金项目： | 国家自然科学基金项目(31071527); 上海市科委重点科技攻关项目(074905114) |
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| 摘 要： | 以萌发期(1～6d)的苦荞芽粉为原料，采用铝盐显色可见分光光度法和HPLC 法，探明萌发期(1～6d)苦荞芽粉乙醇提取物中总黄酮、芦丁和槲皮素含量的变化趋势。以人乳腺癌细胞MCF-7 为模型，采用噻唑蓝(MTT)比色法，比较萌发期(1～6d)的苦荞芽粉乙醇提取物对人乳腺癌细胞体外的增殖抑制率。结果表明：苦荞芽粉乙醇提取物具有抑制MCF-7 乳腺癌细胞增殖的作用，尤以萌发第3 天(芦丁与槲皮素含量比为0.92:1)时抑制效果最好，显示二者具有良好的协同抑制效果；苦荞芽粉乙醇提取物的抑制效果与槲皮素和芦丁标准品模拟样品抑制效果相似，表明苦荞芽粉乙醇提取物对MCF-7 细胞的生长起抑制作用的主要功效成分为槲皮素和芦丁。
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| 关 键 词： | 苦荞 萌发 黄酮 槲皮素 芦丁 MCF- 7 细胞 |
| 收稿时间： | 2010-04-06 |
Inhibitory Effect of Flavonoids from Tartary Buckwheat Sprouts on Proliferation of Human Breast Cancer Cells |
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| ZHOU Xiao-li,WANG Qing,YANG Yan-li,LI Zong-jie,TANG Wen.Inhibitory Effect of Flavonoids from Tartary Buckwheat Sprouts on Proliferation of Human Breast Cancer Cells[J].Food Science,2011,32(1):225-228. |
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| Authors: | ZHOU Xiao-li WANG Qing YANG Yan-li LI Zong-jie TANG Wen |
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| Affiliation: | (1. School of Perfume and Aroma Technology, Shanghai Institute of Technology, Shanghai 200235, China；
2. College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China；
3. College of Life and Environmental Sciences, Shanghai Normal University, Shanghai 200234, China) |
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| Abstract: | The contents of total flavonoids,quercetin and rutin in the powders of tartary buckwheat sprouts germinated for 1 through 6 days were determined using spectrophotometery and HPLC.The anticancer effect of total flavonoids from tartary buckwheat sprouts on human breast cancer MCF-7 cells was evaluated by MTT assay.Results indicated that the ethanol extracts(rich in flavonoids) from all the tartary buckwheat sprouts exhibited an obvious inhibitory effect on the proliferation of MCF-7 cells,especially for the e... |
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| Keywords: | tartary buckwheat germination flavonoids quercetin rutin MCF-7 cell |
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