毛细管电泳法拆分肾上腺素手性对映体

目的：采用毛细管区带电泳法（Capillary Zone Electrophoresis,CZE）,利用β-环糊精（β-cyclodextrin,β-CD）和羟丙基-β-环糊精（Hydroxypropyl-β-cyclodextrin,HP-β-CD）作为手性添加剂,拆分肾上腺素（Epinephrine）手性对映体,并检测市售盐酸肾上腺素注射液的手性状况。方法：通过对缓冲液体系、酸碱性以及β-CD和HP-β-CD的比较研究,得到优化的分离条件,即50mmol/L的Tris-H3PO4（用磷酸调pH为2.42）,含40mmol/L的HP-β-CD,二极管阵列检测器检测,监视波长214nm,分离电压30kV,分离温度25℃,分离时间15min,压力进样5s。结果：肾上腺素（（±）-Epinephrine）手性对映体在HP-β-CD体系中获得了完全的拆分,而β-CD对其没有拆分效果。检测发现市售的盐酸肾上腺素注射液为单一手性化合物。结论：毛细管区带电泳结合HP-β-CD手性添加剂可以有效的拆分肾上腺素手性对映体,并可作为检测产品纯度的有效方法。

Separation of epinephrine enantiomers using Capillary Zone Electrophoresis

Objective：To establish a method based on Capillary Zone Electrophoresis to separate epinephrine enantiomers.Methods：The electrophoretic condition and parameters were investigated,and the optimized conditions were as follow ing：the electrophoretic medium was 50 mmol/L Tris-H3PO4 buffer（pH adjusted to 2.42 with H3PO4） containing 40 mmol/L HP-β-CD as chiral selector.Determination was carried out with detector of DAD at 214 nm.The separation were per formed at 25℃ with a positive voltage of 30 kV.Samples were injected into the capillary by pressure for 5 s.Results：The epinephrine enantiomers were separated entirely when they were in HP-β-CD buffer.Conclusion：CZE combined with HP-β-CD as an adjunct is a valid method to separate the epinephrine enantiomers.