利用丝状噬菌体展示技术进行抗原决定族图谱及其基因工程的研究

噬茵体展示是90年代初发展起来的一种新型表达技术。其主要特点是得到表达的蛋白或肽段能够被展示在病毒粒子的表面，从而使得大规模的专一性选择成为可能。目前此技术已被广泛用于生命科学研究的不同领域。比较突出的有抗体工程的研究，随机抗原决定族库的研究．以及随机肽在新药开发中的研究。本文将集中回顾一下噬菌体展示技术在抗原决定族定位研究中的应用，及其在新型诊断试剂和疫苗开发中的潜在前景。

Application of Filamentous Phage Display Technology to the Mapping and Engineering of Epitopes

: Phage display is a novel expression technology developed in the early 1990's. The major advantage of phage display expression is that the expressed proteins of pep-tides are displayed on the virus surface, which makes large scale spacific selection possible. This new technology is currently being used in may different biologyical research fields, the major ones being antibody engineering, random epitope libraries, and development of new drugs using random peptide libraries. This review will focus on the application of phage display technology in epitope studies and its potential application in the development of novel diagnostic reagents and vaccines.