应用SELDI-TOF-MS技术分析不同中医证型肝癌血清标志物的研究

目的:应用表面加强激光解析电离飞行时间质谱(SELDI-TOF-MS)检测不同中医证型肝癌患者血清蛋白质指纹图谱,筛选特异性相关血清生物学标志物。方法:应用SELDI-TOF-MS蛋白质芯片技术检测5种中医证型(气滞证、脾虚证、湿热证、血瘀证、阴虚证)肝癌患者血清,获得CM10蛋白质芯片蛋白表达图谱,用Marker Wizard软件分析差异蛋白并经数据库搜索初步鉴定。结果:应用弱阳离子交换芯片(CM10)在不同中医证型肝癌血清中检测到在不同质荷比(M/Z)差异蛋白质峰,气滞证组M/Z为4182Da、6589Da及湿热证组M/Z为5710Da的血清蛋白质峰下调,脾虚证组M/Z为5816Da、血瘀证组M/Z为4297Da、阴虚证组M/Z为6992Da的血清蛋白质峰上调,上述蛋白质含量差异均有统计学意义(P0.01)。结论:应用SEIDI-T0F-MS可以筛选不同中医证型肝癌患者血清特异性生物标志物,检测到的差异蛋白质可能是鉴别肝癌不同中医证型的血清生物学指标。

The application of SELDI-TOF-MS technology in analyzing different TCM syndromes of liver cancer serum markers

Objective：Surface-enhanced laser desorption/inionation-time of flight-mass spectrometry（SELDI-TOF-MS） was used to study the serum proteome of hepatocellular carcinoma（HCC） with different syndrome of TCM.Methods：The serum of normal people and patients with different syndrome HCC were collected and tested by CM10 protein chip and SELDI-TOF-MS technology.The data of spectra were analyzed by bioinformatics tools and biomarker pattern discrimination software.Results：Compared with normal group,the peak intensity of M/Z 4182Da、6589Da protein of Qi stagnation syndrome,M/Z 5710Da of Damp heat syndrome were reduced significantly（P0.01）,while the peak intensity of M/Z 5816Da of Spleen deficiency syndrome,M/Z 4297Da of blood stasis syndrome,M/Z 6992Da of Yin deficiency syndrome were increased（P0.01）.Conclusion：These proteins might be related to HCC with different TCM syndrome,and could become candidate biomarkers for clinic differentiation of syndromes in HCC.