传染性支气管炎病毒血凝抗原的制备

目的利用磷脂酶C处理，制备传染性支气管炎病毒（IBV）血凝抗原。方法将IBV接种9日龄SPF鸡胚，48h后收获尿囊液，经过高速离心浓缩，利用胰酶、产气荚膜梭状芽孢杆菌培养液（含磷脂酶C）处理后，进行常规血凝和血凝抑制试验。结果经过胰酶处理的IBV具有非常强的非特异性。而经过磷脂酶C处理的IBV，该凝集作用可被特异的IBV抗血清所抑制，而不能被ILTV、NDV、Adeno-1和Adeno-3，APIV、AIV、IBDV、HP、MS和MG等抗血清所抑制。结论磷脂酶C处理的IBV，可以用于血凝抑制试验。

Preparation of Hemagglutination Antigen to Infectious Bronchitis Virus

Objective To prepare IBV agglutination antigen via treating with phospholipase C. Methods IBV was inoculated into allantoic cavity of 9-day-old SPF chicken embryo. The eggs were incubated at 37℃ for 48 hours. Allantoic fluid was collected and centrifuged at high speed for concentration, following to individually treated with trypsin and clostridium perfringen culture （containing phospholipase C ）. Then performing routine agglutination test and agglutination inhibition test. Results IBV haemagglutination showed strong non-specificity by means of trypsin treatment. On the contrary, IBV haemagglutination expressed powerful specific with phospholipase C because the haemagglutination could be inhibited by specific antiserum to IBV, simultaneously could not be inhibited by antiserum to ILTV, NDV, Adeno-1 and 3, APIV, AIV, IBDV, HP, MS and MG. Conclusion IBV treated with phospholipase C could be used for haemagglutination inhibition test.