不同作用机制杀菌剂对番茄灰霉病菌拮抗木霉菌的毒力测定

目的]为提高木霉的生防效果,筛选出与木霉联用的杀菌剂。方法]对峙法、孢子悬浮液法和离体叶片法测定木霉对灰霉病菌的抑制作用。菌丝生长速率法测定10种不同作用机制的杀菌剂对2个病菌的抑制作用,并用EC50比值>1作为筛选标准。结果]对峙条件下抑制率为69.52%;孢子悬浮液法和离体叶片法的EC50值分别为1.4×108、7.5×1010cfu/L。啶酰菌胺、氟啶胺、嘧菌酯、咯菌腈和啶菌唑对2个病菌的EC50比值>1。结论]上述5种杀菌剂可作为木霉联用防治灰霉病的理想杀菌剂。

Toxicity Measurement of Different Active Mechanism Fungicides to Trichoderma Antagonistic Strain against Botrytis cinerea

by screening out fungicides combined with Trichoderma to improve control effect of Trichoderma on Botrytis cinerea.Methods] The inhibitory effect of Trichoderma on B.cinerea was determined with confrontation,spore suspension and the detached leaf method.The toxicity effect of ten kinds of fungicides with different mechanism to Trichoderma and B.cinerea were tested by mycelium growth rate method,and the ratio value of EC50 beyond 1 was used as the screening standard.Results] Under confrontation method,the inhibition rate of Trichoderma to B.cinerea was 69.52%.The EC50 values by spore suspension method and the detached leaf method were 1.4×108 and 7.5×1010 cfu/L,respectively,the ratio of EC50 of boscalid,fluazinam,azoxystrobin,fludioxonil and pyrisoxazole were more than 1.Conclusions] Fives different fungicides mentioned above are regarded as optional fungicides combined with Trichoderma to control B.cinerea.