康莱特联合阿霉素的体外抗肿瘤活性

目的探讨康莱特与阿霉素联合应用的体外抗肿瘤活性及其相关机制。方法将康莱特与阿霉素单独及联合应用于骨肉瘤细胞,采用MTT法检测细胞毒性作用,流式细胞仪定量分析凋亡细胞所占比例,倒置相差显微镜、荧光显微镜下观察凋亡细胞形态改变。结果康莱特质量浓度达到1 g.L-1时,细胞抑制率不再明显改变,但如与1 mg.L-1阿霉素合用将使细胞抑制率进一步提升(P<0.01)。细胞形态观察及凋亡率测定也显示,康莱特与阿霉素联用可诱导更多骨肉瘤细胞凋亡。结论康莱特与阿霉素合用可高效杀伤肿瘤细胞。

Antitumor activity of Kanglaite combined with doxorubicin in vitro

Objective To study the antitumor activity in vitro of Kanglaite combined with doxorubicin and related mechanism.Methods Kanglaite and doxorubicin were applied on the OS-732 cells respectively or jointly and inhibition rate was measured by MTT assay;The celluar shape were observed with inverted phase contrast microscope and fluorescence microscope;The apoptotic rate was analyzed with flow cytometry(FCM).Results Kanglaite had the effect of up-regulating Fas expression and inducing apoptosis;the inhibition rate was stagnated with 1 g·L~(-1) Kanglaite,if combined with 1 mg·L~(-1) doxorubicin,the inhibition rate was increased obviously(P<0.01).Changes of Celluar shape and apoptotic rate also showed apoptosis-inducing effect of joint application,which was much stronger than those of respective application.Conclusions Combination of Kanglaite and doxorubicin show a strong killing effect on OS-732 cells.