| 重组日本血吸虫SjGST-Sj32蛋白诱导小鼠保护性免疫的研究 |
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| 引用本文： | 蔡春,易新元,曾宪芳,周金春,舒新华.重组日本血吸虫SjGST-Sj32蛋白诱导小鼠保护性免疫的研究[J].中南大学学报(医学版),1999,24(3):225-228. |
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| 作者姓名： | 蔡春 易新元 曾宪芳 周金春 舒新华 |
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| 作者单位： | 湖南医科大学,寄生虫学教研室,长沙,410078 |
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| 基金项目： | WHO／TDR基金,湖南省科委基金 |
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| 摘 要： | 应用基因工程技术分别表达出ＳｊＧＳＴＳｊ３２，Ｓｊ３２和ＳｊＧＳＴ，用于保护性免疫的动物实验及其体液免疫机制的初步探讨。结果：与对照组相比，重组ＳｊＧＳＴＳｊ３２组，Ｓｊ３２组，Ｓｊ３２＋ＳｊＧＳＴ组均有明显的减虫效果（Ｐ＜０．０５）。上述三组及ＳｊＧＳＴ组的肝脏虫卵计数均较对照组为少（Ｐ＜０．０１）。提示重组Ｓｊ３２ｋＤ蛋白可诱导小鼠产生明显的抗日本血吸虫攻击感染的保护性免疫及抗生殖免疫效果，而ＳｊＧＳＴ仅表现出一定的抗生殖免疫效果。
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| 关 键 词： | 日本血吸虫 重组蛋白类 疫苗 免疫 小鼠 |
Schistosoma japonicum: Protective immunity induced in mice immunized with SjGST-Sj32 |
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| Cai Chun,Yi Xinyuan,Zeng Xianfang,Zhou Jinchun,Shu Xinhua.Schistosoma japonicum: Protective immunity induced in mice immunized with SjGST-Sj32[J].Journal of Central South University (Medical Sciences)Journal of Central South University (Medical Sciences),1999,24(3):225-228. |
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| Authors: | Cai Chun Yi Xinyuan Zeng Xianfang Zhou Jinchun Shu Xinhua |
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| Abstract: | Schistosoma
japonicum glutathione s transferase(SjGST), Schistosoma japonicum 32000 protein(Sj32)
and SjGST Sj32 were expressed and purified to study on protective immunity against
Schistosoma japonicum. The result showed that compared with the adjuvant control group, the
worm burden was decreased significantly in mice immunized with recombinant SjGST Sj32,
Sj32 and Sj32+SjGST ( P <0.05). At the same time, the liver egg per gram(EPG) was decreased
in mice immunized with SjGST Sj32, Sj32, Sj32+SjGST and SjGST. It is concluded that Sj32
significantly induces both protective immunity and anti fecundity immunity, but SjGST only has
an anti fecundity effect. |
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| Keywords: | Schistosoma japonicum recombinant proteins vaccine immune mice |
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