| 离子交换层析法分离大鲵乳酸脱氢酶同工酶 |
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| 引用本文： | 马成仓,李清芳.离子交换层析法分离大鲵乳酸脱氢酶同工酶[J].淮北煤炭师范学院学报(自然科学版),1989(2). |
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| 作者姓名： | 马成仓 李清芳 |
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| 作者单位： | 淮北煤炭师范学院，渭南教育学院 |
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| 摘 要： | 采用DEAE—纤维素阴离子交换层析,以0.02M tris-HCl+0.06M NaClpH7.8和0.02M tris-HCl0.15M NaCl pH7.4缓冲液分部洗脱,可以分离大鲵乳酸氢酶同工酶LDH_1和LDH_5。
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| 关 键 词： | 大鲵 乳酸脱氢酶 离子交换层析 |
Isolation of giant salamander laclate dehydrogenate isoenzymes by ion exchange chromatography |
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| Ma Chengcang Li Qingfang.Isolation of giant salamander laclate dehydrogenate isoenzymes by ion exchange chromatography[J].Journal of Huaibei Coal Industry Teachers College(Natural Science edition),1989(2). |
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| Authors: | Ma Chengcang Li Qingfang |
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| Affiliation: | Ma Chengcang Li Qingfang |
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| Abstract: | By using DEAE-cellulose anion exchange chromalography and fractionally eluting with 0.02M tris-HCl+0.06M NaCl pH7.8 and 0.02M tris-HCl+0.15M NaC1 pH7.4 buffer, LDHt and LDH_6 isozmes of giant salamander can be seperated. |
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| Keywords: | giant salamander laclate dehydrogenase ion-exchange chromatography |
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