| 膀胱移行细胞癌患者RASSF1A蛋白表达和启动子区甲基化的研究 |
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| 引用本文： | 胡建庭,李宏召,史涛坪,马鑫,王保军,徐华,艾星,居正华,王超,张国玺,张旭.膀胱移行细胞癌患者RASSF1A蛋白表达和启动子区甲基化的研究[J].临床泌尿外科杂志,2008,23(7). |
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| 作者姓名： | 胡建庭 李宏召 史涛坪 马鑫 王保军 徐华 艾星 居正华 王超 张国玺 张旭 |
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| 作者单位： | 华中科技大学同济医学院附属同济医院泌尿外科,武汉,430030 |
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| 摘 要： | 目的:探讨RASSFlA(RAS association domain family1A)蛋白表达水平与启动子甲基化的关系.方法:应用免疫印迹技术(western-blot)检测10例正常膀胱组织和23例膀胱移行细胞癌(Badder transitional cell carcinoma,BTCC)组织中RASSFlA蛋白表达水平;用甲基化特异性PCR(Methylation-Specific PCR,MSP)方法检测正常膀胱组织,BTCC组织中RASSF1A基因启动子CPG岛甲基化状态.结果:RASSF1A蛋白在正常膀胱组织中全部表达,在BTCC组织中表达率为30.4%(7/23),二组间表达差异有统计学意义(P＜0.05),但各肿瘤分期、病理分级间的表达差异无统计学意义(P＞0.05).在正常膀胱组织中,RASSF1A基因启动子未发生甲基化;在BTCC组织中,RASSF1A基因启动子甲基化频率为60.9%(14/23),二组间表达差异有统计学意义(P＜0.05).在14份启动子异常甲基化的BTCC标本中,13份同时伴有RASSFIA蛋白表达缺失或下调,两者存在明显相关性(P＜0.05).结论:RASSFlA基因启动子在BTCC组织中发生异常甲基化,使RASSF1A蛋白表达缺失,推测RASSFIA基因启动子CPG岛异常甲基化导致RASSF1A基因失活从而引起肿瘤的发生,而与肿瘤的进展可能无关.
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| 关 键 词： | 膀胱移行细胞癌 DNA甲基化 RASSF1A基因 |
The expression of RASSF1A protein and promoter hypermethylation of RASSF1A gene in bladder transitional cell carcinoma |
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| HU Jianting,LI Hong zhao,SHI Taoping,MA Xin,WANG Baojun,XU Hua,AI Xing,JU Zhenghua,WANG Chao,ZHANG Guoxi,ZHANG Xu.The expression of RASSF1A protein and promoter hypermethylation of RASSF1A gene in bladder transitional cell carcinoma[J].Journal of Clinical Urology,2008,23(7). |
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| Authors: | HU Jianting LI Hong zhao SHI Taoping MA Xin WANG Baojun XU Hua AI Xing JU Zhenghua WANG Chao ZHANG Guoxi ZHANG Xu |
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| Affiliation: | 1Department of Urology;Tong ji Hospital;Tong ji Medical College;Huazhong University of Science and Technology;Wuhan;430030;China |
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| Abstract: | Objective:TO investigate the expression of RASSF1A Protein and Promoter hypermethylation of RASSF1A gene in bladder transitional Cell Carcinoma and to analyze the association between them.Methods:RASSF1A Protein expression level was measured by Western-blot in 10 normal bladder tissues and 23 BTCC tissues.The Promoter methylation status in BTCC and normal bladder tissues were tested by methylation-specific PCR.Results:RASSF1A Protein were all Shown in 10 normal bladder tissues and the expression rate is 30.... |
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| Keywords: | Bladder transitional cell carcinoma DNA methylation RAS association domain family1 A gene |
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